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| Content Provider | Springer Nature Link |
|---|---|
| Author | Yang, Jing Li Zhao, Bo Seong, Eun Soo Kim, Myong Jo Kang, Won Hee Kim, Na Young Yu, Chang Yeon Li, Cheng Hao |
| Copyright Year | 2010 |
| Abstract | We describe culture conditions for a high-efficiency in vitro regeneration system of Papaver nudicaule through somatic embryogenesis and secondary somatic embryogenesis. The embryogenic callus induction rate was highest when petiole explants were cultured on Murashige and Skoog (MS) medium containing 1.0 mg l$^{−1}$ α-naphthaleneacetic acid (NAA) and 0.1 mg l$^{−1}$ 6-benzyladenine (BA) (36.7%). When transferred to plant growth regulator (PGR)-free medium, 430 somatic embryos formed asynchronously from 90 mg of embryogenic callus in each 100-ml flask. Early-stage somatic embryos were transferred to MS medium containing 1.0 mg l$^{−1}$ BA and 1.0 mg l$^{−1}$ NAA to germinate at high frequency (97.6%). One-third-strength MS medium with 1.0% sucrose and 1.0 mg l$^{−1}$ GA$_{3}$ had the highest frequency of plantlet conversion from somatic embryos (91.2%). Over 90% of regenerated plantlets were successfully acclimated in the greenhouse. Secondary somatic embryos were frequently induced directly when the excised hypocotyls of the primary somatic embryos were cultured on MS medium without PGRs. Sucrose concentration significantly affected the induction of secondary embryos. The highest induction rate (89.5) and number of secondary somatic embryos per explant (9.3) were obtained by 1% sucrose. Most secondary embryos (87.2–94.3%) developed into the cotyledonary stage on induction medium. All cotyledonary secondary embryos were converted into plantlets both in liquid and on semisolid 1/3-strength MS medium with 1.0% sucrose. |
| Starting Page | 261 |
| Ending Page | 267 |
| Page Count | 7 |
| File Format | |
| ISSN | 18635466 |
| Journal | Plant Biotechnology Reports |
| Volume Number | 4 |
| Issue Number | 4 |
| e-ISSN | 18635474 |
| Language | English |
| Publisher | Springer Japan |
| Publisher Date | 2010-08-03 |
| Publisher Place | Japan |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Papaveraceae Germination Embryogenic callus Somatic embryo somatic embryo Agriculture Biotechnology Plant Biochemistry Cell Biology Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Biotechnology |
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