NDLI: Efficient plant regeneration from immature embryo cultures of Jatropha curcas, a biodiesel plant

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Plant Biotechnology Reports : Volume 4, Issue 1, January 2010

Plant Biotechnology Reports : Volume 3

Plant Biotechnology Reports : Volume 2

Plant Biotechnology Reports : Volume 1

Efficient plant regeneration from immature embryo cultures of Jatropha curcas, a biodiesel plant

Content Provider	Springer Nature Link
Author	Varshney, Alok Johnson, T. Sudhakar
Copyright Year	2010
Abstract	Jatropha curcas L. (Physic nut) is a commercially important non-edible oil seed crop known for its use as an alternate source of biodiesel. In order to investigate the morphogenic potential of immature embryo, explants from four developmental stages were cultured on medium supplemented with combinations of auxins and cytokinins. It was found that the size of embryo is critical for the establishment of callus. Immature embryos (1.1–1.5 cm) obtained from the fruits 6 weeks after pollination showed a good response of morphogenic callus induction (85.7%) and subsequent plant regeneration (70%) with the maximum number of plantlets (4.7/explant) on Murashige and Skoog’s (MS) medium supplemented with IBA (0.5 mg l$^{−1}$) and BA (1.0 mg l$^{−1}$). The above medium when supplemented with growth adjuvants such as 100 mg l$^{−1}$ casein hydrolysate + 200 mg l$^{−1}$l-glutamine + 8.0 mg l$^{−1}$ CuSO$_{4}$ resulted in an even higher frequency of callus induction (100%). Plant regeneration (90%) with the maximum number of plantlets (10/explant) was achieved on MS medium supplemented with 500 mg l$^{−1}$ polyvinyl pyrrolidone + 30 mg l$^{−1}$ citric acid + 1 mg l$^{−1}$ BA + 0.5 mg l$^{−1}$ Kn + 0.25 mg l$^{−1}$ IBA. It was observed that plantlet regeneration could occur either through organogenesis of morphogenic callus or via multiplication of pre-existing meristem in immature embryos. The age of immature embryos and addition of a combination of growth adjuvants to the culture medium appear to be critical for obtaining high regeneration rates. Well-developed shoots rooted on half-strength MS medium supplemented with 0.5 mg l$^{−1}$ IBA and 342 mg l$^{−1}$ trehalose. The rooted plants after acclimatization were successfully transferred to the field in different agro-climatic zones in India. This protocol has been successfully evaluated on five elite lines of J. curcas.
Starting Page	139
Ending Page	148
Page Count	10
File Format	PDF
ISSN	18635466
Journal	Plant Biotechnology Reports
Volume Number	4
Issue Number	2
e-ISSN	18635474
Language	English
Publisher	Springer Japan
Publisher Date	2010-02-21
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Energy crop Immature embryo Jatropha curcas Morphogenesis Plant regeneration Tree-borne oil seed Agriculture Biotechnology Plant Biochemistry Cell Biology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Biotechnology

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