NDLI: Antifouling potential of bacteria isolated from a marine biofilm

Content Provider	Springer Nature Link
Author	Gao, Min Wang, Ke Su, Rongguo Li, Xuzhao Lu, Wei
Copyright Year	2014
Abstract	Marine microorganisms are a new source of natural antifouling compounds. In this study, two bacterial strains, Kytococcus sedentarius QDG-B506 and Bacillus cereus QDG-B509, were isolated from a marine biofilm and identified. The bacteria fermentation broth could exert inhibitory effects on the growth of Skeletonema costatum and barnacle larvae. A procedure was employed to extract and identify the antifouling compounds. Firstly, a toxicity test was conducted by graduated pH and liquid-liquid extraction to determine the optimal extraction conditions. The best extraction conditions were found to be pH 2 and 100% petroleum ether. The EC $_{50}$ value of the crude extract of K. sedentarius against the test microalgae was 236.7 ± 14.08 μg mL$^{−1}$, and that of B. cereus was 290.6 ± 27.11 μg mL$^{−1}$. Secondly, HLB SPE columns were used to purify the two crude extracts. After purification, the antifouling activities of the two extracts significantly increased: the EC $_{50}$ of the K. sedentarius extract against the test microalgae was 86.4 ± 3.71 μg mL$^{−1}$, and that of B. cereus was 92.6 ± 1.47 μg mL$^{−1}$. These results suggest that the metabolites produced by the two bacterial strains are with high antifouling activities and they should be fatty acid compounds. Lastly, GC-MS was used for the structural elucidation of the compounds. The results show that the antifouling compounds produced by the two bacterial strains are myristic, palmitic and octadecanoic acids.
Starting Page	799
Ending Page	804
Page Count	6
File Format	PDF
ISSN	16725182
Journal	Journal of Ocean University of China
Volume Number	13
Issue Number	5
e-ISSN	19935021
Language	English
Publisher	Science Press
Publisher Date	2014-07-09
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	marine biofilm bacteria antifouling activity fatty acids Oceanography Meteorology
Content Type	Text
Resource Type	Article
Subject	Oceanography Ocean Engineering

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