NDLI: Construction of cDNA library from intestine, mesentery and coelomocyte of Apostichopus japonicus Selenka infected with Vibrio sp. and a preliminary analysis of immunity-related genes

Please wait, while we are loading the content...

Spatial-temporal variations of dominant drought/flood modes and the associated atmospheric circulation and ocean events in rainy season over the east of China

Analysis of cost functions for retrieving sea surface salinity

The inhibitory degree between Skeletonema costatum and dinoflagllate Prorocentrum donghaiense at different concentrations of phosphate and nitrate/phosphate ratios

Optimization of sample pretreatment for determination of polycyclic aromatic hydrocarbons in estuarine sediments by gas chromatography

$^{210}$Pb chronology and trace metal geochemistry in the intertidal sediment of Qinjiang River estuary, China

Embryology of Maldives clownfish, Amphiprion nigripes (Amphiprioninae)

Effects of temperature, salinity, light intensity, and pH on the eicosapentaenoic acid production of Pinguiococcus pyrenoidosus

Construction of cDNA library from intestine, mesentery and coelomocyte of Apostichopus japonicus Selenka infected with Vibrio sp. and a preliminary analysis of immunity-related genes

TAT improves in vitro transportation of fortilin through midgut and into hemocytes of white shrimp Litopenaeus vannamei

Properties of polysaccharides in several seaweeds from Atlantic Canada and their potential anti-influenza viral activities

Screening and characterization of the alkaline protease isolated from PLI-1, a strain of Brevibacillus sp. collected from Indonesia’s hot springs

Chitosan oligosaccharide-Ca complex accelerates the depuration of cadmium from Chlamys ferrari

Effects of brown fish meal replacement with fermented soybean meal on growth performance, feed efficiency and enzyme activities of Chinese soft-shelled turtle, Pelodiscus sinensis

Study on antithrombotic and antiplatelet activities of low molecular weight fucoidan from Laminaria japonica

Importance of weighting for multi-variable habitat suitability index model: A case study of winter-spring cohort of Ommastrephes bartramii in the Northwestern Pacific Ocean

Recovery of astaxanthin from discharged wastewater during the production of chitin

A study on marine region carrying capacity and eco-compensation

Journal of Ocean University of China : Volume 11, Issue 1, March 2012

Journal of Ocean University of China : Volume 10

Journal of Ocean University of China : Volume 9

Journal of Ocean University of China : Volume 8

Journal of Ocean University of China : Volume 7

Journal of Ocean University of China : Volume 6

Journal of Ocean University of China : Volume 5

Journal of Ocean University of China : Volume 4

Journal of Ocean University of China : Volume 3

Journal of Ocean University of China : Volume 2

Journal of Ocean University of China : Volume 1

Construction of cDNA library from intestine, mesentery and coelomocyte of Apostichopus japonicus Selenka infected with Vibrio sp. and a preliminary analysis of immunity-related genes

Content Provider	Springer Nature Link
Author	Liu, Hongzhan Zheng, Fengrong Sun, Xiuqin Cai, Yimei
Copyright Year	2012
Abstract	The aquaculture of sea cucumber Apostichopus japonicus (Echinodermata, Holothuroidea) has grown rapidly during recent years and has become an important sector of the marine industry in Northern China. However, with the rapid growth of the industry and the use of non-standard culture techniques, epidemic diseases of A. japonicus now pose increasing problems to the industry. To screen the genes with stress response to bacterial infection in sea cucumber at a genome wide level, we constructed a cDNA library from A. japonicus Selenka (Aspidochirotida: Stichopodidae) after infecting them with Vibrio sp. for 48 h. Total RNA was extracted from the intestine, mesentery and coelomocyte of infected sea cucumber using Trizol and mRNA was isolated by Oligotex mRNA Kits. The ligated cDNAs were transformed into DH5α, and a library of 3.24×10$^{5}$ clones (3.24×10$^{5}$ cfu mL$^{−1}$) was obtained with the sizes of inserted fragments ranging from 0.8 to 2.5 kb. Sequencing the cDNA clones resulted in a total of 1106 ESTs that passed the quality control. BlastX and BlastN searches have identified 168 (31.5%) ESTs sharing significant homology with known sequences in NCBI protein or nucleotide databases. Among a panel of 25 putative immunity-related genes, serum lectin isoform, complement component 3, complement component 3-like genes were further studied by real-time PCR and they all increased more than 5 fold in response to Vibrio sp. challenge. Our library provides a valuable molecular tool for future study of invertebrate immunity against bacterial infection and our gene expression data indicates the importance of the immune system in the evolution and development of sea cucumber.
Starting Page	187
Ending Page	196
Page Count	10
File Format	PDF
ISSN	16725182
Journal	Journal of Ocean University of China
Volume Number	11
Issue Number	2
e-ISSN	19935021
Language	English
Publisher	SP Science Press
Publisher Date	2012-03-03
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Apostichopus japonicus cDNA library expressed sequence tags immunity-related genes real-time PCR Oceanography
Content Type	Text
Resource Type	Article
Subject	Oceanography Ocean Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effects of dietary β-glucan and glycyrrhizin on non-specific immunity and disease resistance of the sea cucumber (Apostichopus japonicus Selenka) challenged with Vibrio splendidus

Comparative analysis of genes expressed in regenerating intestine and non-eviscerated intestine of Apostichopus japonicus Selenka (Aspidochirotida: Stichopodidae) and cloning of ependymin gene

Identification and characterization of 43 microsatellite markers derived from expressed sequence tags of the sea cucumber (Apostichopus japonicus)

Growth, metabolism and physiological response of the sea cucumber, Apostichopus japonicus Selenka during periods of inactivity

Construction of cDNA library of Pyrocystis lunula (Pyrophyta)

Influence of dietary probiotic Bacillus TC22 and Prebiotic fructooligosaccharide on growth, immune responses and disease resistance against Vibrio splendidus infection in sea cucumber Apostichopus japonicus

Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda

Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger

Stress responses to rapid temperature changes of the juvenile sea cucumber (Apostichopus japonicus Selenka)

Construction of cDNA library from intestine, mesentery and coelomocyte of Apostichopus japonicus Selenka infected with Vibrio sp. and a preliminary analysis of immunity-related genes

Similar Documents

Effects of dietary β-glucan and glycyrrhizin on non-specific immunity and disease resistance of the sea cucumber (Apostichopus japonicus Selenka) challenged with Vibrio splendidus

Comparative analysis of genes expressed in regenerating intestine and non-eviscerated intestine of Apostichopus japonicus Selenka (Aspidochirotida: Stichopodidae) and cloning of ependymin gene

Identification and characterization of 43 microsatellite markers derived from expressed sequence tags of the sea cucumber (Apostichopus japonicus)

Growth, metabolism and physiological response of the sea cucumber, Apostichopus japonicus Selenka during periods of inactivity

Construction of cDNA library of Pyrocystis lunula (Pyrophyta)

Influence of dietary probiotic Bacillus TC22 and Prebiotic fructooligosaccharide on growth, immune responses and disease resistance against Vibrio splendidus infection in sea cucumber Apostichopus japonicus

Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda

Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger

Stress responses to rapid temperature changes of the juvenile sea cucumber (Apostichopus japonicus Selenka)

Construction of cDNA library from intestine, mesentery and coelomocyte of Apostichopus japonicus Selenka infected with Vibrio sp. and a preliminary analysis of immunity-related genes