NDLI: Comparative analysis of genes expressed in regenerating intestine and non-eviscerated intestine of Apostichopus japonicus Selenka (Aspidochirotida: Stichopodidae) and cloning of ependymin gene

Content Provider	Springer Nature Link
Author	Zheng, Fa Xin Sun, Xiu Qin Fang, Bao Hai Hong, Xu Guang Zhang, Jin Xing
Copyright Year	2006
Abstract	The regeneration of the intestine of sea cucumber (Apostichopus japonicus) was studied by describing historically the changes that occurred during intestine regeneration on the fifth day after chemically-induced evisceration. An expressed sequence tag (EST) analysis was undertaken to identify major genes, which might be involved in intestine regeneration of A. japonicus. Two cDNA libraries were constructed with directional cloning method, one for regenerating intestine collected on the third, fourth and fifth day after evisceration (post-evisceration, PE), and the other for the non-eviscerated (NE). A total of 730 ESTs were generated by sequencing cDNA clones from the two libraries (372 from PE and 358 from NE). The results showed that the number of genes that were involved in primary metabolism of PE library was less than that of NE library, while the number of genes involved in cell defense/immunity, cell division, cell signal transduction/communication of PE library was more than that of NE library. The results also revealed that the expression of the genes which might be involved in regeneration was enhanced to some extent after evisceration. Only about 11.54% of the sequenced clones were shared by two libraries, which provided some clues for the existence of differential gene expression between PE and NE intestines. A gene named epenAj was also characterized in this study.
Starting Page	109
Ending Page	122
Page Count	14
File Format	PDF
ISSN	00188158
Journal	Hydrobiologia
Volume Number	571
Issue Number	1
e-ISSN	15735117
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-07-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Apostichopus japonicus ependymin encoding gene expressed sequence tag regenerating intestine regeneration associated gene Ecology Hydrobiology
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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