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| Content Provider | Springer Nature Link |
|---|---|
| Author | Cai, Chuner Wang, Yuan Li, Chunxia Guo, Ziye Jia, Rui Wu, Weining Hu, Yan He, Peimin |
| Copyright Year | 2014 |
| Abstract | Phycoerythrin and phycocyanin were purified from Porphyra yezoensis Ueda with their bioactivity determined in this study. Continuous precipitation with ammonium sulfate at different concentrations (10%, 20%, 40% and 50%) increased the purity (A$_{564}$:A$_{280}$) of phycoerythrin to 1.49, 3.92 fold of the raw extract (0.38) and the purity (A$_{615}$:A$_{280}$) of phycocyanin to 0.70, 3.33 fold of the raw extract (0.21). Two more times of chromatography with hydroxylapatites finally made the purity of phycoerythrin and phycocyanin reach 5.50, 14.47 fold of the raw extract, and 5.10, 24.29 fold of the raw extract, respectviely. The yield of high purity phycoerythrin and phycocyanin were 0.21% and 0.09% of dried P. yezoensis blade, respectively. The photodynamic cytotoxic experiment showed that both phycoerythrin and phycocyanin inhibited the growth of liver tumor cells significantly. It was found that 250 mg L$^{−1}$ purified phycoerythrin and phycocyanin inhibited the growth of hepatocellular carcinoma cells 24 h after laser-irradiation by 80% and 59%, respectively, and 100 mg L$^{−1}$ purified phycoerythrin and phycocyanin induced the apoptosis of 31.54% and 32.54% of the cells, respectively, 8 h after photodynamic therapy. Oue findings demonstrated that P. yezoensis can serve as photosensitizer (phycoerythrin and phycocyanin) producer. |
| Starting Page | 479 |
| Ending Page | 484 |
| Page Count | 6 |
| File Format | |
| ISSN | 16725182 |
| Journal | Journal of Ocean University of China |
| Volume Number | 13 |
| Issue Number | 3 |
| e-ISSN | 19935021 |
| Language | English |
| Publisher | Science Press |
| Publisher Date | 2014-05-10 |
| Publisher Place | Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Porphyra yezoensis phycoerythrin phycocyanin purification photosensitivity apoptosis hepatocellular carcinoma cell Oceanography Meteorology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Oceanography Ocean Engineering |
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