Purification, crystallization and preliminary X-ray analysis of phycocyanin and phycoerythrin from Porphyra yezoensis Ueda.

Content Provider	Semantic Scholar
Author	Cai, Chuner Wu, Lian Ming Li, Chunxia He, Peimin Zhou, Jiahai
Copyright Year	2011
Abstract	Porphyra yezoensis is one of the most important and widely cultured seaweeds in China. Phycobiliproteins exhibit excellent spectroscopic properties and play versatile roles in the biomedical, food, cosmetics and chemical synthetic dye industries. Here, the purification and crystallization of phycoerythrin and phycocyanin, two phycobiliproteins extracted from P. yezoensis, are described. Using a novel protocol including co-precipitation with ammonium sulfate and hydroxyapatite column chromatography, both phycobiliproteins were produced on a large scale with improved quality and yield compared with those previously reported. Native PAGE analysis indicated that phycoerythrin and phycocyanin exist as (αβ)(3) heterohexamers in solution. The crystals of phycoerythrin diffracted to 2.07 Å resolution and belonged to space group R3. The unit-cell parameters referred to hexagonal axes are a = b = 187.7, c = 59.7 Å, with nine (αβ)(2) heterotetramers per unit cell. The crystals of phycocyanin diffracted to 2.70 Å resolution in space group P2(1). Matthews coefficient analysis shows that 10-19 (αβ) heterodimers of phycocyanin in the asymmetric unit would be reasonable. A self-rotation function calculation clarified this ambiguity and indicated that 12 (αβ) heterodimers of phycocyanin are assembled in the asymmetric unit.
File Format	PDF HTM / HTML
DOI	10.1107/S1744309111007925
Alternate Webpage(s)	http://journals.iucr.org/f/issues/2011/05/00/fw5295/fw5295.pdf
PubMed reference number	21543866
Alternate Webpage(s)	https://doi.org/10.1107/S1744309111007925
Journal	Medline
Volume Number	67
Part	5
Journal	Acta crystallographica. Section F, Structural biology and crystallization communications
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article