NDLI: Adsorption equilibria of fatty acids between methanol/water and reversed-phase chromatographic adsorbents

Content Provider	Springer Nature Link
Author	Ib ez Gonz lez, M. J. Medina, A. Robles Esteban Cerd n, L. Camacho P ez, B. Gim nez Gim nez, A. Grima, E. Molina
Copyright Year	2001
Abstract	The adsorption equilibria are discussed for fatty acids 16∶1n−7, 16∶2n−4, and 20∶5n−3 (eicosapentaenoic acid, EPA). These fatty acids are major components of a polyunsaturated fatty acid concentrate from the microalga Phaeodactylum tricornutum. The solvents used were methanol/water (1% acetic acid) mixtures of different compositions, and the adsorbents used were chromatographic reversed-phases octylsilyl C$_{8}$, octadecylsilyl C$_{18}$, and dodecylsilyl C$_{22}$ of different particle and pore sizes. The kinetic studies showed that equilibrium was attained instantaneously, suggesting an absence of mass transfer limitations. The equilibrium data were fitted by the Freundlich isotherm. The separation efficiency of EPA from 16∶1n−7 and 16∶2n−4 in all the adsorbent-solvent systems was compared in terms of the separation factors α$_{EPA/16:2n-4}$=K $_{EPA}$/K $_{16:2n-4}$ and α$_{16:1n-7/EPA}$=K $_{16:1n-7}$/K $_{EPA}$, where K $_{ i }$ is the fatty acid distribution ratio between the stationary and the liquid phases. The EPA separation from 16∶1n−7 and 16∶2n−4 by liquid chromatography could be predicted using the Craig model for the various solvent-adsorbent combinations. The best adsorbents for purifying EPA were: C$_{18}$, PEP, 8 μm, 100 Å, and C$_{22}$, 10 μm, 100 Å, and the best solvent was methanol/water (1% acetic acid) 75∶25, w/w.
Starting Page	277
Ending Page	285
Page Count	9
File Format	PDF
ISSN	0003021X
Journal	Journal of the American Oil Chemists' Society
Volume Number	78
Issue Number	3
e-ISSN	15589331
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Adsorption isotherms Craig model eicosapentaenoic acid reversed-phase chromatographic adsorbents Chemistry/Food Science Analytical Chemistry Biotechnology Industrial Chemistry/Chemical Engineering Biomaterials Agriculture
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Chemical Engineering

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