NDLI: Optimization of fatty acid extraction from Phaeodactylum tricornutum UTEX 640 biomass

Content Provider	Springer Nature Link
Author	Ibáñez González, M. J. Robles Medina, A. Grima, E. Molina Giménez, A. Giménez Carstens, M. Cerdán, L. Esteban
Copyright Year	1998
Abstract	Fatty acids in the microalga Phaeodactylum tricornutum were isolated using an optimized three-step method: extraction of crude fatty acid potassium salts made by direct saponification of lipids in the microalgal biomass with KOH/ethanol (96%, vol/vol), separation of unsaponifiable lipids by extraction with hexane, and final purification of fatty acids by acidification of the alcoholic solution of potassium soaps followed by extraction of fatty acid into hexane. Direct saponification was carried out in ethanol (96%, vol/vol) using 2.09 mL ethanol (96%) per gram of wet biomass (10 mL/g of dry biomass) mixed with 0.4 g KOH/g of biomass. Under these conditions the fatty acid yield was 87%. The optimal water content of the alcoholic solution for extraction of the unsapononifiables was established as 40%, w/w. Data on equilibrium carotenoid distribution between the alcoholic (40%, w/w water) and hexane phases were determined. These data allow prediction of the carotenoid yields with different volumes of hexane in several extraction steps. The optimal pH of the alcoholic solution before extracting the purified fatty acid was established as pH 6, and the equilibrium fatty acid distribution between the alcoholic and hexane phases was determined. This optimized method permited a 20% reduction in the production costs of highly purified eicosapentaenoic acid (EPA) in the three-step preparative process (extraction of fatty acid, concentration of polyunsaturated fatty acids by the urea method, and EPA fractionation through preparative high-performance liquid chromatography) previously developed by the authors.
Starting Page	1735
Ending Page	1740
Page Count	6
File Format	PDF
ISSN	0003021X
Journal	Journal of the American Oil Chemists' Society
Volume Number	75
Issue Number	12
e-ISSN	15589331
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Eicosapentaenoic acid (EPA) fatty acid extraction fatty acid purification Phaeodactylum tricornutum Industrial Chemistry/Chemical Engineering Analytical Chemistry Chemistry/Food Science Biotechnology Biomaterials Agriculture
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Chemical Engineering

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