NDLI: Simultaneous determination of sucrose and trehalose in olive leaves by spectrophotometry utilizing partial least squares method

Content Provider	Springer Nature Link
Author	Khanmohammadi, Mohammadreza Moeini, Maryam Bagheri Garamarudi, Amir Sotudehnia, Abbas Zarrabi, Mahdi
Copyright Year	2009
Abstract	This study deals with the simultaneous determination of sucrose and trehalose in olive leaf samples, with easy sample preparation step, by second derivative UV–Vis spectrophotometry and partial least squares technique. A training set consisting of 31 binary mixture solutions was applied for the construction of PLS model. The proposed procedure was successfully applied for the simultaneous determination of both carbohydrates in laboratory prepared mixtures and in real samples. The real samples were from different growth stages of olive plant. The root mean square error of prediction was determined to be 1.95 and 2.06 for sucrose and trehalose, respectively. Also, limit of detection was 0.21 ppm for sucrose and 0.26 ppm for trehalose. The proposed procedure is rapid, simple, and easy to perform which can be used for routine analysis of both carbohydrates in plant leaves.
Starting Page	865
Ending Page	869
Page Count	5
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	31
Issue Number	4
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-03-28
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Olive leaf Sucrose Trehalose Quantification UV spectrophotometry Partial least square Agriculture Plant Anatomy/Development Plant Pathology Plant Biochemistry Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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