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| Content Provider | Springer Nature Link |
|---|---|
| Author | Singh, Shashi Kant Rai, Maj K. Asthana, Pooja Sahoo, L. |
| Copyright Year | 2009 |
| Abstract | An efficient and improved in vitro propagation system for Spilanthes acmella L. using transverse thin cell layer (tTCL) culture system was established. The frequency of shoot regeneration from tTCL nodal segments was affected by concentrations of plant growth regulators and orientation of the explant. MS (Murashige and Skoog in Physiol Plant 15:473–497, 1962) medium with 5.0 mg dm$^{−3}$ BAP was optimal for shoot regeneration. Upon this medium, the explant inoculated in the upright orientation exhibited a high frequency of shoot regeneration (about 97%), and the highest number of shoots (31.5) per explant. The intact node (1.0–1.5 cm) cultured on the same medium had significantly lower shoot multiplication ability with only 4.5 shoots per responsive explant. As compared to BAP alone, the combination of BAP and Kin or NAA did not have positive effects on shoot multiplication from tTCL nodal segments. Rooting of shoots was achieved on growth regulator free full-strength MS medium. Plantlets were transplanted into soil with 90–100% survival rate. |
| Starting Page | 693 |
| Ending Page | 698 |
| Page Count | 6 |
| File Format | |
| ISSN | 01375881 |
| Journal | Acta Physiologiae Plantarum |
| Volume Number | 31 |
| Issue Number | 4 |
| e-ISSN | 18611664 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2009-02-12 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | In vitro propagation Organogenesis Plant growth regulators Spilanthes acmella Thin cell layer explant Agriculture Plant Anatomy/Development Plant Pathology Plant Biochemistry Plant Genetics & Genomics Plant Physiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Plant Science Agronomy and Crop Science |
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