NDLI: Transformation of PttKN1 gene to cockscomb

Content Provider	Springer Nature Link
Author	Meng, Lai Sheng Ding, Wei Qiao Hu, Xin Wang, Chong Ying
Copyright Year	2009
Abstract	We have established a shoot regeneration system and genetic transformation of cockscomb (Celosia cristata and Celosia plumosus). The best results in terms of frequency of shoot regeneration and number of shoot buds per explant are observed on media supplemented with 0.5 mg l$^{−1}$ 6-BA (for explants of apical meristems of C. cristata) or 2.0 mg l$^{−1}$ 6-BA, 0.5 mg l$^{−1}$ NAA and 0.5 mg l$^{−1}$ IAA (for hypocotyls explants of C. plumosus). We use apical meristems of C. cristata and hypocotyls of C. plumosus as the starting material for transformation. A novel KNOTTED1-like homeobox1 (KNOX), PttKN1 (Populus tremula × P. tremuoides knotted1) isolated from the vascular cambial region of hybrid aspen, is introduced into cockscomb by Agrobacterium. A series of novel phenotypes are obtained from the transgenic cockscomb plants, including lobed or rumpled leaves, partite leaves and two or three leaves developed on the same petiole, on the basis of their leaf phenotypes. Transformants are selected by different concentrations of kanamycin. Transformants are confirmed by PCR of the NptII gene and PCR or RT-PCR of PttKN1 gene. Furthermore, RT-PCR shows that 35S:: PttKN1 RNA levels do not correlate with phenotypic severity. It is discussed that our results bring elements on possible function of PttKN1 gene. To our knowledge, genetic transformation of cockscomb is first reported.
Starting Page	683
Ending Page	691
Page Count	9
File Format	PDF
ISSN	01375881
Journal	Acta Physiologiae Plantarum
Volume Number	31
Issue Number	4
e-ISSN	18611664
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-02-13
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cockscomb PttKN1 gene Transgenic plants NptII gene PCR RT-PCR Agriculture Plant Anatomy/Development Plant Pathology Plant Biochemistry Plant Genetics & Genomics Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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