NDLI: An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

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An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

Content Provider	Springer Nature Link
Author	Gantait, Saikat Mandal, Nirmal Bhattacharyya, Somnath Das, Prakash Kanti
Copyright Year	2010
Abstract	A novel, efficient, and simple protocol was developed on in vitro mass propagation and acclimatization of Gerbera jamesonii Bolus cv. Sciella, an ornamental plant with attractive flowers. Shoot tip was used as the primary explant for in vitro establishment in which Murashige and Skoog (MS) medium supplemented with a low level of NAA (0.5 mg l$^{−1}$) and BAP (1.5 mg l$^{−1}$) promoted earliest axillary bud initiation within 5 d in 91.6% of the inoculants. Five axillary buds were initiated from a single explant within 13 d after inoculation. A very high rate of shoot multiplication (14 shoots per inoculated axillary bud) and proliferation was achieved when MS medium was fortified with a relatively higher level of BAP (2 mg l$^{−1}$) and 60 mg l$^{−1}$ ADS within 27 d of multiple shoot culture. A maximum number of well-developed roots per plant was observed in MS medium with 0.5 mg l$^{−1}$ IAA in the next 26 d. In the easy low-cost acclimatization process of 20 d, a combination of sand, soil, cow urine, and tea leaves extract (1:1:1:1; v/v) ensured 95% survival rate. Sixty-one well-acclimatized plants were obtained from a single shoot tip within 86 d. The sustained multiple shoot culture for 15 mo paved the way toward the conservation of genetic resources as well as beneficial economics. The clonal fidelity study of micropropagated and sustained cultured clones using ISSR primers ensured the continuous supply of quality propagules retaining genetic uniformity. The in vitro-generated plants performed better over conventionally propagated plants in the field condition.
Starting Page	537
Ending Page	548
Page Count	12
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	46
Issue Number	6
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-11-05
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Acclimatization Commercial scale Field performance Gerbera ISSR Micropropagation Sustained culture Plant Genetics & Genomics Plant Breeding/Biotechnology Developmental Biology Cell Biology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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