NDLI: Novel micropropagation and weaning methods for the integrated conservation of a critically endangered tree species, Medusagyne oppositifolia

Content Provider	Springer Nature Link
Author	Marriott, Poppy Sarasan, Viswambharan
Copyright Year	2010
Abstract	Medusagyne oppositifolia is a critically endangered monotypic species with only a few trees left in the wild. Due to invasion and habitat clearance, genetic diversity of this species has been reduced to an alarmingly low level. Long-term in vitro cultures of M. oppositifolia were slow-growing and eventually lost the ability to multiply and root. Because of the lack of new seed and vegetative materials, efforts were made to rejuvenate the existing cultures. This was attempted by a series of steps involving serial pruning of the shoot tips and growing on a modified MS medium containing silver nitrate and thidiazuron. Rapid cycling clonal propagules were produced this way from cultured nodes. Isolated shoots were rooted on Florialite™, and rooted plants were weaned in a transition stage using a Nutrient Film Technique (NFT) hydroponic system before being completely weaned under glasshouse conditions. The importance of screening cultures for multiplication and rooting efficiency during long-term maintenance is discussed in the context of stress metabolite production and its influence on growth in culture. Factors contributing to rejuvenation of old cultures, application of unconventional supporting systems for rooting and application of the hydroponic system as a weaning tool are also discussed.
Starting Page	516
Ending Page	523
Page Count	8
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	46
Issue Number	6
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-10-26
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	In vitro Long-term culture Rejuvenation Repatriation Biodiversity Hotspot Plant Genetics & Genomics Plant Breeding/Biotechnology Developmental Biology Cell Biology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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