NDLI: Simultaneous determination and quantification of three pentacyclic triterpenoids—betulinic acid, oleanolic acid, and ursolic acid

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Simultaneous determination and quantification of three pentacyclic triterpenoids—betulinic acid, oleanolic acid, and ursolic acid—in cell cultures of Lantana camara L.

Content Provider	Springer Nature Link
Author	Srivastava, Priyanka Chaturvedi, Rakhi
Copyright Year	2010
Abstract	A simple procedure has been described for simultaneous determination and improved yield of three pentacyclic triterpenoids—betulinic, oleanolic, and ursolic acids—from callus cultures of Lantana camara. Cell biomass was obtained from leaf disk explants cultured on Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium supplemented with 5 μM 6-benzylaminopurine, 1 μM 2,4-dichlorophenoxyacetic acid, and 1 μM α-naphthaleneacetic acid. Optimum separation of the three compounds was achieved by reverse-phase high-pressure liquid chromatography on a C$_{18}$ column with 80:20 (v/v) acetonitrile/water as mobile phase. With this route, a yield of 3.1% betulinic acid, 1.88% oleanolic acid, and 4.12% ursolic acid per gram dry weight was obtained from cultures. Leaves from the parent plant, used as control, showed total absence of betulinic acid, and the quantities of oleanolic and ursolic acids present in them were only marginally higher than that found in in vitro-raised cultures. Presence of the three compounds was further confirmed by electrospray ionization mass spectrometry.
Starting Page	549
Ending Page	557
Page Count	9
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	46
Issue Number	6
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-07-23
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Betulinic acid Leaf cultures Mass spectrometry Oleanolic acid RP-HPLC Ursolic acid Plant Genetics & Genomics Plant Breeding/Biotechnology Developmental Biology Cell Biology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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