NDLI: The establishment and characterization of immortal hepatocyte cell lines from a mouse liver injury model

Content Provider	Springer Nature Link
Author	Risal, Prabodh Cho, Baik Hwan Sylvester, Karl G. Kim, Jae Chun Kim, Hyoung Tae Jeong, Yeon Jun
Copyright Year	2011
Abstract	Hepatocytes are an important research tool used for numerous applications. However, a short life span and a limited capacity to replicate in vitro limit the usefulness of primary hepatocyte cultures. We have hypothesized that in vivo priming of hepatocyte could make them more susceptible to growth factors in the medium for continuous proliferation in vitro. Here, a novel approach used to establish hepatocyte cell lines that included hepatocyte priming in vivo prior to culture with a 3,5-diethoxycarbonyl-1,4-dihydrocollidine diet was attempted. The cell line grew in a monolayer while maintaining a granular cytoplasm and a round nucleus. Electron microscopy displayed hepatocyte-like features including mitochondria, glycogen granules, and the presence of bile canaliculi. This cell line expressed many mature hepatocyte-specific genes including albumin, alpha1-antitrypsin, glucose 6-phosphatase, and tyrosine aminotransferase. Functional characteristic of hepatocytes like the ability to store glycogen, lipid, and synthesis of urea is well demonstrated by this cell line. These cells demonstrated anchorage dependent growth properties in soft agar and did not form tumors after transplantation into nude mice. This cell line can be sustained in culture for more than 100 passages (>1.5 years) without undergoing noticeable morphological changes or transformation. This novel method resulted in the establishment of an immortal, non-transformed hepatocyte cell line with functional characteristics that may aid research of cell metabolism, toxicology, and hepatocyte transplantation.
Starting Page	526
Ending Page	534
Page Count	9
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	47
Issue Number	8
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-07-30
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	In vivo priming Hepatocyte cell line Mouse Immortal hepatocyte Animal Genetics and Genomics Cell Biology Cell Culture Stem Cells Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Establishment and characterization of feeder cell-dependent bovine fetal liver cell lines

Feeder-independent continuous culture of the PICM-19 pig liver stem cell line

Establishment of a human neonatal hepatocyte cell line

Establishment and characterization of a fibroblast cell line from the Mongolian horse

Establishment and characterization of human bladder cancer cell lines BexBra1, BexBra2, and BexBra4

Isolation of multilineage progenitors from mouse brain

Establishment of immortalized murine mesothelial cells and a novel mesothelioma cell line

The establishment and characterization of immortal hepatocyte cell lines from a mouse liver injury model.

Establishment and characterization of two fetal fibroblast cell lines from the yak

The establishment and characterization of immortal hepatocyte cell lines from a mouse liver injury model

Similar Documents

Establishment and characterization of feeder cell-dependent bovine fetal liver cell lines

Feeder-independent continuous culture of the PICM-19 pig liver stem cell line

Establishment of a human neonatal hepatocyte cell line

Establishment and characterization of a fibroblast cell line from the Mongolian horse

Establishment and characterization of human bladder cancer cell lines BexBra1, BexBra2, and BexBra4

Isolation of multilineage progenitors from mouse brain

Establishment of immortalized murine mesothelial cells and a novel mesothelioma cell line

The establishment and characterization of immortal hepatocyte cell lines from a mouse liver injury model.

Establishment and characterization of two fetal fibroblast cell lines from the yak

The establishment and characterization of immortal hepatocyte cell lines from a mouse liver injury model