NDLI: Optimization of human Th17 cell differentiation in vitro: evaluating different polarizing factors

Content Provider	Springer Nature Link
Author	Ganjalikhani Hakemi, Mazdak Ghaedi, Kamran Andalib, Alireza Hosseini, Mohsen Rezaei, Abbas
Copyright Year	2011
Abstract	Regarding discrepancies that exist among different studies which have tried to clarify critical factors in human Th17 cell differentiation, the aim of this study was to identify the best condition for human Th17 differentiation and to clarify the possible role of TGF-β in differentiation of these cells. Naïve CD4$^{+}$ T cells were isolated from cord blood samples and cultured either in X-VIVO 15 serum-free medium or RPMI 1640 containing 10% FBS. Purified cells were treated with different combinations of polarizing cytokines (TGF-β, IL-1β, IL-6, IL-23 and IL-21) followed by analysis of the expression of characteristic genes and their relevant cytokines by real-time quantitative RT-PCR and ELISA method, respectively. Our data indicate that a combination of TGF-β plus IL-6 and IL-23 cytokines in X-VIVO 15 serum-free medium could be applied as the best condition for developing human Th17 cells in compare with other studied cytokine treatments. It is shown that TGF-β could be considered as a positive regulator for human Th17 cell differentiation only if applied in average concentrations. Interestingly, polarizing treatments in absence of TGF-β, induced double-secreting Th17 cells which co-express IL-17 and IFN-γ whereas polarization in presence of TGF-β-induced single-secreting (only IL-17 expressing) Th17 cells.
Starting Page	581
Ending Page	592
Page Count	12
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	47
Issue Number	8
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-08-19
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	IL-17 RORC2 TGF-β Th17 Animal Genetics and Genomics Cell Biology Cell Culture Stem Cells Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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