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  1. In Vitro Cellular & Developmental Biology - Animal
  2. In Vitro Cellular & Developmental Biology - Animal : Volume 37
  3. In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 7, July 2001
  4. Analysis of three marine fish cell lines by RAPD assay
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In Vitro Cellular & Developmental Biology - Animal : Volume 53
In Vitro Cellular & Developmental Biology - Animal : Volume 52
In Vitro Cellular & Developmental Biology - Animal : Volume 51
In Vitro Cellular & Developmental Biology - Animal : Volume 50
In Vitro Cellular & Developmental Biology - Animal : Volume 49
In Vitro Cellular & Developmental Biology - Animal : Volume 48
In Vitro Cellular & Developmental Biology - Animal : Volume 47
In Vitro Cellular & Developmental Biology - Animal : Volume 46
In Vitro Cellular & Developmental Biology - Animal : Volume 45
In Vitro Cellular & Developmental Biology - Animal : Volume 44
In Vitro Cellular & Developmental Biology - Animal : Volume 43
In Vitro Cellular & Developmental Biology - Animal : Volume 42
In Vitro Cellular & Developmental Biology - Animal : Volume 41
In Vitro Cellular & Developmental Biology - Animal : Volume 40
In Vitro Cellular & Developmental Biology - Animal : Volume 39
In Vitro Cellular & Developmental Biology - Animal : Volume 38
In Vitro Cellular & Developmental Biology - Animal : Volume 37
In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 10, November 2001
In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 9, October 2001
In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 8, September 2001
In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 7, July 2001
An in vitro model for murine ureteric epithelial cells
Polyunsaturated fatty acids are not essential for survival and proliferation of a human B cell line
Nonviral gene transfer into primary cultures of human and procine mesothelial cells
Isolation of endothelial cells from human placental microvessels: Effect of different proteolytic enzymes on releasing endothelial cells from villous tissue
Cell motility in a new single-cell wound model
Expansion and long-term culture of differentiated normal rat urothelial cells in vitro
Analysis of three marine fish cell lines by RAPD assay
Production of bioengineered cancer tissue constructs in vitro: Epithelium-mesenchyme heterotypic interactions
Effects of fibroblast growth factor 2 and insulin-like growth factor II on the development of parthenogenetic mouse embryos in vitro
An in vitro method for recording the electrical activity of the isolated heart of the adult Drosophila melanogaster
Differing sensitivity of tumor cells to apoptosis induced by iron deprivation in vitro
Mutations in the human homologue of the Drosophila segment polarity gene Patched in oral squamous cell carcinoma cell lines
Identification of a region on the adenovirus E1a gene responsible for induction by phorbol ester tumor promoter
In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 6, June 2001
In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 5, May 2001
In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 4, April 2001
In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 3, March 2001
In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 2, February 2001
In Vitro Cellular & Developmental Biology - Animal : Volume 37, Issue 1, January 2001
In Vitro Cellular & Developmental Biology - Animal : Volume 36
In Vitro Cellular & Developmental Biology - Animal : Volume 35
In Vitro Cellular & Developmental Biology - Animal : Volume 34
In Vitro Cellular & Developmental Biology - Animal : Volume 33

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Analysis of three marine fish cell lines by RAPD assay

Content Provider Springer Nature Link
Author Guo, Hua Rong Zhang, Shi Cui Tong, Shang Liang Xiang, Jian Hai
Copyright Year 2001
Abstract We tested the applicability of the random amplified polymorphic deoxyribonucleic acid (RAPD) analysis for identification of three marine fish cell lines FG, SPH, and RSBF, and as a possible tool to detect cross-contamination. Sixty commercial 10-mer RAPD primers were tested on the cell lines and on samples collected from individual fish. The results obtained showed that the cell lines could be identified to the correspondent species on the basis of identical patterns produced by 35–48% of the primers tested; the total mean similarity indices for cell lines versus correspondent species of individual fish ranged from 0.825 to 0.851, indicating the existence of genetic variation in these cell lines in relation to the species of their origin. Also, four primers, which gave a monomorphic band pattern within species/line, but different among the species/line, were obtained. These primers can be useful for identification of these cell lines and for characterization of the genetic variation of these cell lines in relation to the species of their origin. This supported the use of RAPD analysis as an effective tool in species identification and cross-contamination test among different cell lines.
Starting Page 430
Ending Page 433
Page Count 4
File Format PDF
ISSN 10712690
Journal In Vitro Cellular & Developmental Biology - Animal
Volume Number 37
Issue Number 7
e-ISSN 1543706X
Language English
Publisher Springer-Verlag
Publisher Date 2001-01-01
Publisher Place New York
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword fish cell line characterization genetic variation random amplified polymorphic DNA Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type Text
Resource Type Article
Subject Cell Biology Developmental Biology Medicine
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