NDLI: Mutations in the human homologue of the Drosophila segment polarity gene Patched in oral squamous cell carcinoma cell lines

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Mutations in the human homologue of the Drosophila segment polarity gene Patched in oral squamous cell carcinoma cell lines

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Mutations in the human homologue of the Drosophila segment polarity gene Patched in oral squamous cell carcinoma cell lines

Content Provider	Springer Nature Link
Author	Michimukai, Eiji Kitamura, Naoya Zhang, Yan Wang, Hua Hiraishi, Yoshiko Sumi, Ken Saku Hayashido, Yasutaka Toratani, Shigeaki Okamoto, Tetsuji
Copyright Year	2001
Abstract	In the present study, we have analyzed tumor deoxyribonucleic acid from oral squamous cell carcinoma (OSCC) cells for patched mutations using an exon-by-exon single strand conformation polymorphism assay and direct sequencing. We found two missense mutations which affected the conserved residue in the transmembrane domains of the gene product and in the intracellular loop at the C-terminal residue implicated in regulating the smoothened molecule. In addition, we demonstrated that the N-terminal fragment of sonic hedgehog (Shh-N) stimulates the growth of normal epithelial cells, the OSCC cell line, NA, and the salivary gland adenocarcinoma cell lines, HSG and HSY, which have no detectable mutation in patched. On the other hand, Shh has no effect on human SCC cells (UE, KA, KO, NI, A431 cells) that have mutations in patched. These results strongly suggest that an Shh-patched signaling is involved in the cell growth of oral epithelial cells and in the tumorigenesis of OSCCs.
Starting Page	459
Ending Page	464
Page Count	6
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	37
Issue Number	7
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	sonic hedgehog patched oral squamous cell carcinoma cells human salivary adenocarcinoma cells mutation Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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