NDLI: Glycated type 1 collagen induces endothelial dysfunction in culture

Content Provider	Springer Nature Link
Author	Kuo, Pei Ching Kao, Cheng Heng Chen, Jan Kan
Copyright Year	2007
Abstract	Hyperglycemia-induced protein glycation is thought to be implicated in the diabetic vasculopathy. In this study, we cultured vascular endothelial cells on native or glycated collagen matrix and compared their growth and functional characteristics. At lower plating density, the cells grew equally well on both substrata; however, at higher planting density, the cells plated on glycated collagen grew slower and reached a lower confluent density compared to that of the native collagen-based cultures. Confluent cell layers formed on glycated collagen exhibited a lower diffusion barrier function and a less response to epidermal growth factor stimulated prostacyclin production, compared to their native collagen-cultured counterparts.
Starting Page	338
Ending Page	343
Page Count	6
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	43
Issue Number	10
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-10-03
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hyperglycemia Prostacyclin EGF Animal Physiology Animal Biochemistry Animal Genetics and Genomics Developmental Biology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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Glycated type 1 collagen induces endothelial dysfunction in culture