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  1. In Vitro Cellular & Developmental Biology - Animal
  2. In Vitro Cellular & Developmental Biology - Animal : Volume 38
  3. In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 4, April 2002
  4. Isolation and culture of rat microvascular endothelial cells
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In Vitro Cellular & Developmental Biology - Animal : Volume 53
In Vitro Cellular & Developmental Biology - Animal : Volume 52
In Vitro Cellular & Developmental Biology - Animal : Volume 51
In Vitro Cellular & Developmental Biology - Animal : Volume 50
In Vitro Cellular & Developmental Biology - Animal : Volume 49
In Vitro Cellular & Developmental Biology - Animal : Volume 48
In Vitro Cellular & Developmental Biology - Animal : Volume 47
In Vitro Cellular & Developmental Biology - Animal : Volume 46
In Vitro Cellular & Developmental Biology - Animal : Volume 45
In Vitro Cellular & Developmental Biology - Animal : Volume 44
In Vitro Cellular & Developmental Biology - Animal : Volume 43
In Vitro Cellular & Developmental Biology - Animal : Volume 42
In Vitro Cellular & Developmental Biology - Animal : Volume 41
In Vitro Cellular & Developmental Biology - Animal : Volume 40
In Vitro Cellular & Developmental Biology - Animal : Volume 39
In Vitro Cellular & Developmental Biology - Animal : Volume 38
In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 10, November 2002
In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 9, October 2002
In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 8, September 2002
In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 7, July 2002
In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 6, June 2002
In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 5, May 2002
In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 4, April 2002
Bladder carcinoma cell line ECV304 is not a model system for endothelial cells
Response to the report by Drexler et al.
A method for purifying enteric glia from rat myenteric plexus
Spontaneous differentiation of porcine and bovine embryonic stem cells (Epiblast) into astrocytes or neurons
The specificity of phenotypic induction of mouse and human stem cells by signaling complexes
Lipotropes regulate BCL-2 gene expression in the human breast cancer cell line, MCF-7
Isolation and culture of rat microvascular endothelial cells
A countermeasure to ameliorate immune dysfunction in in vitro simulated microgravity environment: Role of cellular nucleotide nutrition
Characterization of glucose transport by cultured rabbit kidney proximal convoluted and proximal straight tubule cells
An in vitro model of the back of the eye for studying retinal pigment epithelial-choroidal endothelial interactions
Rb localization and phosphorylation kinetics correlate with the cellular phenotype of cultured breast adenocarcinoma cells
Regression models for overdispersed jejunal surviving crypts data
Sphingosine-1-phosphate stimulates human Caco-2 intestinal epithelial proliferation via p38 activation and activates ERK by an independent mechanism
In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 3, March 2002
In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 2, February 2002
In Vitro Cellular & Developmental Biology - Animal : Volume 38, Issue 1, January 2002
In Vitro Cellular & Developmental Biology - Animal : Volume 37
In Vitro Cellular & Developmental Biology - Animal : Volume 36
In Vitro Cellular & Developmental Biology - Animal : Volume 35
In Vitro Cellular & Developmental Biology - Animal : Volume 34
In Vitro Cellular & Developmental Biology - Animal : Volume 33

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Isolation and culture of rat microvascular endothelial cells

Content Provider Springer Nature Link
Author Frye, Cynthia A. Patrick, Charles W.
Copyright Year 2002
Abstract The purpose of this study is to identify the separation techniques that result in pure cultures of rat microvascular endothelial cells (MECs). A multistep process is used to optimize the separation of the cells from rat epididymal fat pads, obtaining as pure a culture as possible within a relatively short processing time. The process initially employs the digestion, filtration, and density gradient separation steps. We further describe the use of an attachment phase that allows the differential adherence of contaminating cell types. Immunomagnetic purification is the final step in the process and is performed using anti-PECAM-1 (CD31) monoclonal antibody-labeled DynaBeads.
Starting Page 208
Ending Page 212
Page Count 5
File Format PDF
ISSN 10712690
Journal In Vitro Cellular & Developmental Biology - Animal
Volume Number 38
Issue Number 4
e-ISSN 1543706X
Language English
Publisher Springer-Verlag
Publisher Date 2002-01-01
Publisher Place New York
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword cell culture CD31 rat endothelial cells immunomagnetic separation Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type Text
Resource Type Article
Subject Cell Biology Developmental Biology Medicine
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