NDLI: Establishment and cellular characteristics of a hepatocyte cell line (oums-31) derived from an acatalasemic mouse

Content Provider	Springer Nature Link
Author	Kondo, Asami Miyazaki, Masahiro Pu, Hong Gao, Chong Namba, Masayoshi
Copyright Year	1999
Abstract	Liver cell lines with very low catalase activity were established from an acatalasemic mouse. Hepatocytes isolated by a collagenase-liver-perfusion technique were cultured in Williams’ E medium supplemented with 10% fetal bovine serum. The acatalasemic liver cell line showed approximately 20% of the catalase activity of a normal mouse liver cell line, whereas its glutathione peroxidase activity was approximately equal to that of the normal liver cell line. DNA sequence analysis of this cell line showed the same mutation in the catalase gene as is seen in the acatalasemic mouse. Our observation of intracellular content of hydrogen peroxide (H$_{2}$O$_{2}$) radical and increased susceptibility of the cells to H$_{2}$O$_{2}$ were compatible with the existence of low catalase activity in the acatalasemic mouse. This hepatocyte cell line should be useful for studying effects of oxidative radical stress at the cellular level.
Starting Page	155
Ending Page	158
Page Count	4
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	35
Issue Number	3
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	acatalasemia hepatocytes catalase hydrogen peroxide Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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