NDLI: Liver-specific physiology of immortal, functionally differentiated hepatocytes and of deficient hepatocyte-like variants

Content Provider	Springer Nature Link
Author	Priesner, Christoph Hesse, Friedemann Windgassen, Dirk Klocke, Rainer Paul, Dieter Wagner, Roland
Copyright Year	2004
Abstract	Five different immortalized transgenic hepatocyte cell lines derived from mice were investigated with respect to their potential to maintain the physiological properties of primary hepatocytes using chemically defined medium. This research completes a previous study by Klocke and coworkers in 2002, using gene expression analysis of the same cell lines by the respective physiological analysis for investigating the hepatocyte-like function. Three transgenic cell lines harboring a fusion gene derivative (construct 202), consisting of the complete SV40 early region, including the coding sequences for the transforming large and small tumor antigens, placed under the control of the murine metallothioneine 1-promotor/ enhancer element, showed a hepatocyte-like function and physiology. They grew as a monolayer with a polygonal cell shape, consumed lactate, and secreted albumin at a cell-specific rate of 1.5 pg/h, which is in the range of primary hepatocytes. In addition, the potential of detoxifying ammonium could be maintained. Ammonium was metabolized and urea was produced and released into the medium. A complete urea cycle could be determined. A cell line established from neonatal transgenic mice and expressing a secretory variant of the human epidermal growth factor (IgEGF) under the control of the albumin promoter was characterized by an incomplete urea cycle. Another cell line isolated from the liver of homozygote neonatal p53-knockout mice showed no hepatocyte-specific functions but only properties of continuous cell lines. Specific nucleoside triphosphate (NTP) and uridine (U) ratios were used to characterize the differentiation status of the particular cell lines. A low NTP-U value was found for the thre cell lines containing construct 202, which was identical to that observed for primary hepatocytes. In contrast, the cell line harvested from the liver of homozygote neonatal p53-knockout mice presented a NTP-U ratio characteristic for continuous cell lines. This study demonstrates that the four transgenic and the p53-knockout, hepatocyte-derived cell lines can be used as models for investigating the conservation of tissue-specific functions in immortalized cells.
Starting Page	318
Ending Page	330
Page Count	13
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	40
Issue Number	10
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	hepatocyte-like cell lines metabolism nucleotide ratios immortalization Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The establishment and characterization of immortal hepatocyte cell lines from a mouse liver injury model

Establishment of a human neonatal hepatocyte cell line

Immortalization of human hepatocytes by temperature-sensitive SV40 large-T antigen

Establishment and characterization of immortalized ovine sertoli cell lines

Three-dimensional culture of hepatocytes in a continuously flowing medium

SV40 large T antigen immortalization of rat hepatic stellate-like cells

Modulation of mitogen-independent hepatocyte proliferation during the perinatal period in the rat

Elevated expression of hormone-regulated rat hepatocyte functions in a new serum-free hepatocyte-stromal cell coculture model

Establishment of uterine cell lines from p53-deficient mice

Liver-specific physiology of immortal, functionally differentiated hepatocytes and of deficient hepatocyte-like variants

Similar Documents

The establishment and characterization of immortal hepatocyte cell lines from a mouse liver injury model

Establishment of a human neonatal hepatocyte cell line

Immortalization of human hepatocytes by temperature-sensitive SV40 large-T antigen

Establishment and characterization of immortalized ovine sertoli cell lines

Three-dimensional culture of hepatocytes in a continuously flowing medium

SV40 large T antigen immortalization of rat hepatic stellate-like cells

Modulation of mitogen-independent hepatocyte proliferation during the perinatal period in the rat

Elevated expression of hormone-regulated rat hepatocyte functions in a new serum-free hepatocyte-stromal cell coculture model

Establishment of uterine cell lines from p53-deficient mice

Liver-specific physiology of immortal, functionally differentiated hepatocytes and of deficient hepatocyte-like variants