NDLI: Suppressive effects of fructus of Magnolia denudata on IL-4 and IL-13 expression in T cells

Content Provider	Springer Nature Link
Author	Jin, Mirim Kim, Soon Rye Yoon, Soo Jeong Jeong, Hwa Hyun Kim, Dae Keun Cho, Eun Yang, Mihi Pyo, Myoung Yun
Copyright Year	2013
Abstract	Magnolia species have been used for the treatment of allergic diseases in Asia as folk medicine; however, the cellular and molecular mechanisms of its anti-allergic effects have rarely been investigated. In this study, we demonstrated that a methanolic extract of the fructus of Magnolia denudata has suppressive effects on Th2 cytokine production such as IL-4 and IL-13, but not IFN-γ and IL-17, produced by both phorbol 12-myristate 13-acetate/ionomycin (PI)- and CD3/CD28-stimulated EL-4 T cells. Moreover, the mRNA expression of Th2 cytokines was significantly inhibited, and luciferase activity in cells transiently transfected with IL-4 or IL-13 promoter reporter plasmids was suppressed by M. denudata, indicating that M. denudata may regulate these expression at the transcriptional level. Western blot analysis for transcription factors involved in the cytokine gene expression indicated that the activation of c-Jun was significantly downregulated in the nucleus of cells, while the activations of nuclear factor of activated T cells, nuclear factor kappa B and c-Fos, were not affected. Furthermore, the mRNA expression and nuclear translocation of GATA-binding protein 3, a key transcriptional factor for Th2 commitment and Th2 cytokine expression, but not T-bet and RORγt, were dramatically downregulated by M. denudata. Treatment with M. denudata suppressed the phosphorylation of p38 mitogen-activated protein kinase; however, the PI-induced phosphorylation of extracellular signal-related kinase and c-Jun N-terminal kinase was unaffected. Taken together, our study indicated that M. denudata inhibited IL-4 and IL-13 expression, possibly through regulation of p38 mitogen-activated protein kinase phosphorylation and selective transcription factors, such as GATA-3 and c-Jun, in EL-4 T cells.
Starting Page	805
Ending Page	814
Page Count	10
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	49
Issue Number	10
e-ISSN	1543706X
Language	English
Publisher	Springer US
Publisher Date	2013-08-16
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anti-allergic agent IL-4 IL-13 Magnolia denudata Transcription factors Cell Biology Developmental Biology Stem Cells Cell Culture Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Suppressive effects of fructus of Magnolia denudata on IL-4 and IL-13 expression in T cells.

Expression pattern and functional role of Phc2 during activation of helper T cells after antigenic stimulation

Primary culture of insect midgut cells ( In Vitro Cellular & Developmental Biology - Animal , Volume 45 , Issue 3-4 )

Expression and quantification of Oct-4 gene in blastocyst and embryonic stem cells derived from in vitro produced buffalo embryos

Modeling the airway epithelium in allergic asthma: Interleukin-13-induced effects in differentiated murine tracheal epithelial cells

Optimization of human Th17 cell differentiation in vitro: evaluating different polarizing factors

Aneuploidy in immortalized human mesenchymal stem cells with non-random loss of chromosome 13 in culture

Animal symposia

Animal posters

Suppressive effects of fructus of Magnolia denudata on IL-4 and IL-13 expression in T cells

Similar Documents

Suppressive effects of fructus of Magnolia denudata on IL-4 and IL-13 expression in T cells.

Expression pattern and functional role of Phc2 during activation of helper T cells after antigenic stimulation

Primary culture of insect midgut cells ( In Vitro Cellular & Developmental Biology - Animal , Volume 45 , Issue 3-4 )

Expression and quantification of Oct-4 gene in blastocyst and embryonic stem cells derived from in vitro produced buffalo embryos

Modeling the airway epithelium in allergic asthma: Interleukin-13-induced effects in differentiated murine tracheal epithelial cells

Optimization of human Th17 cell differentiation in vitro: evaluating different polarizing factors

Aneuploidy in immortalized human mesenchymal stem cells with non-random loss of chromosome 13 in culture

Animal symposia

Animal posters

Suppressive effects of fructus of Magnolia denudata on IL-4 and IL-13 expression in T cells