NDLI: Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats

Content Provider	Springer Nature Link
Author	Karimata, Toru Sato, Daisuke Seya, Daiki Sato, Daichi Wakatsuki, Takashi Feng, Zhonggang Nishina, Atsuyosi Kusuki, Masataka Nakamura, Takao
Copyright Year	2013
Abstract	Reconstructed myocardial tissue still does not have enough pulsatile contraction. It is well known that fetal and mature neonatal cardiomyocytes utilize glucose and lipid, respectively, as their energy substrates, and that cultured ones mainly use glucose in spite of their age comparable to neonate ones, probably due to insufficient supply of lipids from culture medium. In the present study, we compared 7 saturated, 6 monounsaturated, and 11 polyunsaturated fatty acid contents in cultured cardiomyocytes (Cul group) with those in fetal (Fet group, approximately 17 d after impregnation) and neonatal (Neo group, 9 d old) rats, where the age of the Cul cells were set nearly equal to the Neo ones. Saturated fatty acid contents in the Cul group were generally lower than those in the Fet group and were close to those in the Neo group, except for C12:0 of which content was highest in the Neo group. Monounsaturated fatty acid contents in the Cul group were generally lower than those in the Fet group but similar to or higher than those in the Neo group, except for C24:1n-9 of which content was again highest in the Neo group. In contrast, most of polyunsaturated fatty acid (PUFA) contents in the Cul group appeared lower than those in both the Fet and Neo groups, and differences in 5 of 10 detected PUFAs were significant between the Cul and Neo groups. The results suggest that PUFA contents in cultured cardiomyocytes might be insufficient to exert enough contractile ability. In conclusion, it could be necessary for cultured cardiomyocytes to uptake more lipid; PUFAs in particular.
Starting Page	798
Ending Page	804
Page Count	7
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	49
Issue Number	10
e-ISSN	1543706X
Language	English
Publisher	Springer US
Publisher Date	2013-08-16
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cardiomyocytes Fatty acid composition Energy substrate Cardiac tissue engineering Cell Biology Developmental Biology Stem Cells Cell Culture Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Erratum to: Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats

A fetal human heart cardiac-inducing RNA (CIR) promotes the differentiation of stem cells into cardiomyocytes

Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats.

Tissue-engineered fetal dermal matrices

Differential Effects of Hypoxic and Hyperoxic Stress-Induced Hypertrophy in Cultured Chick Fetal Cardiac Myocytes

Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes

Neonatal rat heart cells cultured in simulated microgravity

Culture of amelanotic melanocytes derived from human fetal hair follicles

Polyunsaturated fatty acid metabolism in enterocyte models: T84 cell line vs. Caco-2 cell line

Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats

Similar Documents

Erratum to: Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats

A fetal human heart cardiac-inducing RNA (CIR) promotes the differentiation of stem cells into cardiomyocytes

Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats.

Tissue-engineered fetal dermal matrices

Differential Effects of Hypoxic and Hyperoxic Stress-Induced Hypertrophy in Cultured Chick Fetal Cardiac Myocytes

Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes

Neonatal rat heart cells cultured in simulated microgravity

Culture of amelanotic melanocytes derived from human fetal hair follicles

Polyunsaturated fatty acid metabolism in enterocyte models: T84 cell line vs. Caco-2 cell line

Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats