NDLI: Isolation and characterization of a Klebsiella pneumoniae strain from mangrove sediment for efficient biosynthesis of 1,3-propanediol

Content Provider	Springer Nature Link
Author	Zhou, Sheng Li, Lili Perseke, Marleen Huang, Youhua Wei, Jingguang Qin, Qiwei
Copyright Year	2015
Abstract	1,3-Propanediol (PDO) is an important bulk industrial material. It can be produced by microbial fermentation. In this study, the microbial flora of mangrove sediment was screened to identify strains with high production of PDO by fermentation of glycerol. The PDO productivities of the isolated strains were tested, and the strain with highest PDO productivity was characterized using the API20E and 16-s rRNA sequence analysis. The physiological and phylogenetic analysis indicated that the strain was closed related to K. pneumoniae species and was named as K. pneumoniae HSL4. The structure of the dha cluster which was responsible for the biosynthesis of PDO was analyzed. It is observed that K. pneumoniae HSL4 was tolerant to salt and partly tolerant to acetate and lactate, which will favor industrial applications. Fed-batch fermentation experiments revealed K. pneumoniae HSL4 exhibited an excellent ability to produce PDO with high concentration (80.08 g L$^{−1}$), productivity (2.22 g L$^{−1}$h$^{−1}$) and conversion (0.435 g g$^{−1}$ or 0.53 mol mol$^{−1}$). The metabolic flux profile illuminated that glycerol was consumed rapidly and PDO was accumulated quickly to a high level during the exponential growth phase. This study provided important information for further fermentation and metabolic engineering of PDO production by K. pneumoniae HSL4.本研究的目的是从海洋环境中分离鉴定用于发酵生产1,3-丙二醇的海洋微生物菌株。1,3-丙二醇是一种重要的工业原料,主要用于生产新型聚酯材料——聚对苯二甲酸丙二醇脂。通过富集与选择培养、摇瓶发酵测试,筛选得到一株高产1,3-丙二醇菌株;通过API20E试剂条反应、16S rRNA基因序列比对分析、电镜形态观察及相关基因簇分析,鉴定命名该菌株为Klebsiella pneumoniae HSL4。进一步分析表明该菌株具有耐盐、耐有机酸的特征。补料发酵实验表明该菌株合成1,3-丙二醇浓度、生产强度、转化率分别达到80.08 g L$^{−1}$, 2.22 g L$^{−1}$h$^{−1}$, 0.53 mol mol$^{−1}$ 。本研究首次报道了从海洋沉积物环境中分离得到高产1,3-丙二醇的菌株。
Starting Page	511
Ending Page	521
Page Count	11
File Format	PDF
ISSN	20959273
Journal	Chinese Science Bulletin
Volume Number	60
Issue Number	5
e-ISSN	20959281
Language	Chinese
Publisher	Science China Press
Publisher Date	2015-02-13
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Marine Klebsiella pneumoniae Fermentation 1,3-Propanediol Metabolism Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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