NDLI: Development of cystathionine gamma-lyase-specific microRNAs

Content Provider	Springer Nature Link
Author	Li, Shuangshuang Yang, Guangdong
Copyright Year	2015
Abstract	The gasotransmitter role of H$_{2}$S in mammalian has been extensively studied, and cystathionine gamma-lyase (CSE) is the major H$_{2}$S-producing enzyme in vascular system. Dysregulation of CSE/H$_{2}$S system was found in various pathophysiological conditions. MicroRNA (miRNA) and short interfering RNA (siRNA) are known to inhibit gene expression by mRNA degradation and/or translation repression. The regulation of CSE expression by miRNA and siRNA has been reported recently, but the off-target effect of miRNA and the lower knockdown efficiency of siRNA have shadowed the application of these approaches. In the present study, we designed CSE-specific miRNAs based on the rules of miRNA–mRNA complementary and human CSE cDNA sequence. The CSE-specific miRNAs significantly inhibited CSE expression and H$_{2}$S production, increased reactive oxygen species generation, and induced proliferation of human aorta smooth muscle cells (HASMCs). The designed CSE-specific miRNAs specifically targeted on CSE gene as evidenced by the direct inhibition of luciferase activity from reporter gene containing human CSE 3′-UTR sequence. The expression of other genes, such as estrogen receptor α, heme oxygenase 1, specificity protein 1, and 3-mercaptopyruvate sulfurtransferase, was not affected by the CSE-specific miRNAs. Compared with CSE-siRNAs, CSE-specific miRNAs displayed significantly higher efficacy in suppressing CSE expression and H$_{2}$S production. miR-143, a highly expressed miRNA in vascular system, down-regulated the expressions of CSE as well as other genes, such as insulin-like growth factor binding protein 5 and kruppel-like factor 4. miR-143 suppressed H$_{2}$S production but had no effect on HASMC proliferation. In conclusion, CSE-specific miRNAs designed in our study provide a highly effective research tool for regulating CSE expression and H$_{2}$S production. These CSE-specific miRNAs have potential as being novel therapeutic agents for treating vascular disorders related to abnormal oxidative stress and SMC growth.
Starting Page	503
Ending Page	510
Page Count	8
File Format	PDF
ISSN	20959273
Journal	Chinese Science Bulletin
Volume Number	60
Issue Number	5
e-ISSN	20959281
Language	English
Publisher	Science China Press
Publisher Date	2015-02-15
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	H$_{2}$S Cystathionine gamma-lyase MicroRNAs siRNA Smooth muscle cells Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Deficiency of cystathionine gamma-lyase and hepatic cholesterol accumulation during mouse fatty liver development

Reflection on microRNAs in cancer: the next decade

Plant development and reproduction

Autophagy in mitotic animal cells

Extracellular ATP mediates H$_{2}$S-regulated stomatal movements and guard cell K$^{+}$ current in a H$_{2}$O$_{2}$-dependent manner in Arabidopsis

Modeling of solid oxide fuel cells

Zic1 negatively regulates brown adipogenesis in C$_{3}$H$_{10}$T$_{1/2}$ cells

H$_{2}$S synthetase AtD-CDes involves in ethylene and drought regulated stomatal movement

Specificity Protein-1 as a Critical Regulator of Human Cystathionine γ-Lyase in Smooth Muscle Cells

Development of cystathionine gamma-lyase-specific microRNAs

Similar Documents

Deficiency of cystathionine gamma-lyase and hepatic cholesterol accumulation during mouse fatty liver development

Reflection on microRNAs in cancer: the next decade

Plant development and reproduction

Autophagy in mitotic animal cells

Extracellular ATP mediates H$_{2}$S-regulated stomatal movements and guard cell K$^{+}$ current in a H$_{2}$O$_{2}$-dependent manner in Arabidopsis

Modeling of solid oxide fuel cells

Zic1 negatively regulates brown adipogenesis in C$_{3}$H$_{10}$T$_{1/2}$ cells

H$_{2}$S synthetase AtD-CDes involves in ethylene and drought regulated stomatal movement

Specificity Protein-1 as a Critical Regulator of Human Cystathionine γ-Lyase in Smooth Muscle Cells

Development of cystathionine gamma-lyase-specific microRNAs