NDLI: Homologous and heterologous interactions between catalytic and regulatory subunits of Escherichia coli acetohydroxyacid synthase I and III

Content Provider	Springer Nature Link
Author	Niu, CongWei Feng, Wei Zhou, YanFei Wen, Xin Xi, Zhen
Copyright Year	2009
Abstract	Homologous and heterologous interactions between acetohydroxyacid synthase (AHAS) I and III from E. coli have been studied by surface plasmon resonance (SPR). The catalytic and regulatory subunits association for AHAS I (K $_{D}$ = 1.13 × 10$^{−7}$ M) was stronger than that for AHAS III (K $_{D}$ = 5.29 × 10$^{−7}$ M). A strong heterologous association between regulatory and catalytic subunits and heterologous activation of catalytic subunits were observed. SPR results combined with enzyme kinetics indicate that the reconstituted heterologous enzymes had similar kinetic properties as homologous enzymes, implying that the regulatory subunit of AHAS I could be replaced by the regulatory subunit of AHAS III and vice versa. This work may be useful to further understandings of the mechanism of regulation of AHAS.
Starting Page	1362
Ending Page	1371
Page Count	10
File Format	PDF
ISSN	10069291
Journal	Science in China Series B: Chemistry
Volume Number	52
Issue Number	9
e-ISSN	18622771
Language	English
Publisher	SP Science in China Press
Publisher Date	2009-09-24
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	acetohydroxyacid synthase subunit interaction surface plasmon resonance Chemistry/Food Science
Content Type	Text
Resource Type	Article
Subject	Chemistry

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