NDLI: Separation/enrichment of the low-content high molecular weight natural protein using protein-imprinted polymers with ARPCs

Content Provider	Springer Nature Link
Author	Xia, JianJun Long, Yi Guo, MinJie Wang, Ying Mi, HuaiFeng
Copyright Year	2009
Abstract	We introduce a new method for separation/enrichment of the low-content cellular protein in high molecular weight on the basis of molecular imprinting. The template protein, bacterial cloned immunoglobulin binding protein (BiP), was selectively assembled with assistant recognition polymer chains (ARPCs) from their library, which consists of numerous limited length polymer chains with randomly distributed recognition and immobilizing sites. The assemblies of proteins and ARPCs were adsorbed by porous polymeric beads and immobilized by cross-linking polymerization. After the template was removed, the synthesized imprinted polymer was used to adsorb authentic BiP from endoplasmic reticulum (ER) extract, and its proportional content was enriched 45 times. It is the first time that the low-content cellular natural protein, whose molecular weight reaches 78 kDa, is enriched by molecular imprinting.
Starting Page	1388
Ending Page	1393
Page Count	6
File Format	PDF
ISSN	10069291
Journal	Science in China Series B: Chemistry
Volume Number	52
Issue Number	9
e-ISSN	18622771
Language	English
Publisher	SP Science in China Press
Publisher Date	2009-09-24
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	molecular imprinting technique assistant recognition polymer chains (ARPCs) bacterial cloned protein high molecular weight protein Chemistry/Food Science
Content Type	Text
Resource Type	Article
Subject	Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in