NDLI: Rat submandibular glands secrete nanovesicles with NTPDase and 5′-nucleotidase activities

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Purinergic Signalling : Volume 10

Purinergic Signalling : Volume 9

Purinergic Signalling : Volume 8

Purinergic Signalling : Volume 7

Purinergic Signalling : Volume 6

Purinergic Signalling : Volume 5

Purinergic Signalling : Volume 4

Purinergic Signalling : Volume 3

Purinergic Signalling : Volume 2

Purinergic Signalling : Volume 1

Rat submandibular glands secrete nanovesicles with NTPDase and 5′-nucleotidase activities

Content Provider	Springer Nature Link
Author	González, Débora A. Egido, Patricia Balcarcel, elia B. Hattab, Claude Barbieri van Haaster, Martín M. Pelletier, Julie Sévigny, Jean Ostuni, Maria A.
Copyright Year	2014
Abstract	Extracellular nucleotides modulate a wide number of biological processes such as neurotransmission, platelet aggregation, muscle contraction, and epithelial secretion acting by the purinergic pathway. Nucleotidases as NTPDases and ecto-5′-nucleotidase are membrane-anchored proteins that regulate extracellular nucleotide concentrations. In a previous work, we have partially characterized an NTPDase-like activity expressed by rat submandibular gland microsomes, giving rise to the hypothesis that membrane NTPDases could be released into salivary ducts to regulate luminal nucleotide concentrations as was previously proposed for ovarian, prostatic, and pancreatic secretions. Present results show that rat submandibular glands incubated in vitro release membrane-associated NTPDase and ecto-5′-nucleotidase activities. Electron microscopy images show that released membranes presenting nucleotidase activity correspond to exosome-like vesicles which are also present at microsomal fraction. Both exosome release and nucleotidase activities are raised by adrenergic stimulation. Nucleotidase activities present the same kinetic characteristics than microsomal nucleotidase activity, corresponding mainly to the action of NTPDase2 and NTPDase3 isoforms as well as 5′-nucleotidase. This is consistent with Western blot analysis revealing the presence of these enzymes in the microsomal fraction.
Starting Page	107
Ending Page	116
Page Count	10
File Format	PDF
ISSN	15739538
Journal	Purinergic Signalling
Volume Number	11
Issue Number	1
e-ISSN	15739546
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-12-19
Publisher Institution	International Purine Club
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Submandibular gland Extracellular nucleotides NTPDases Nucleotidases Nanovesicles Extracellular vesicles Biomedicine general Pharmacology/Toxicology Human Physiology Neurosciences Cancer Research
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Cellular and Molecular Neuroscience

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Rat submandibular glands secrete nanovesicles with NTPDase and 5′-nucleotidase activities

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Rat submandibular glands secrete nanovesicles with NTPDase and 5′-nucleotidase activities

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Rat submandibular glands secrete nanovesicles with NTPDase and 5′-nucleotidase activities