NDLI: Pharmacological and molecular characterization of functional P2 receptors in rat embryonic cardiomyocytes

Please wait, while we are loading the content...

Mutation in the Drosophila melanogaster adenosine receptor gene selectively decreases the mosaic hyperplastic epithelial outgrowth rates in wts or dco heterozygous flies

Rat submandibular glands secrete nanovesicles with NTPDase and 5′-nucleotidase activities

Purine receptors are required for DHA-mediated neuroprotection against oxygen and glucose deprivation in hippocampal slices

Pharmacological and molecular characterization of functional P2 receptors in rat embryonic cardiomyocytes

Shear stress modulates endothelial KLF2 through activation of P2X4

NTPDase2 and the P2Y$_{1}$ receptor are not required for mammalian eye formation

Purinergic Signalling : Volume 10

Purinergic Signalling : Volume 9

Purinergic Signalling : Volume 8

Purinergic Signalling : Volume 7

Purinergic Signalling : Volume 6

Purinergic Signalling : Volume 5

Purinergic Signalling : Volume 4

Purinergic Signalling : Volume 3

Purinergic Signalling : Volume 2

Purinergic Signalling : Volume 1

Pharmacological and molecular characterization of functional P2 receptors in rat embryonic cardiomyocytes

Content Provider	Springer Nature Link
Author	Cheung, Kwok Kuen Marques da Silva, Camila Vairo, Leandro Santos, Danúbia Silva Goldenberg, Regina Coutinho Silva, Robson Burnstock, Geoffrey
Copyright Year	2014
Abstract	Purinergic receptors activated by extracellular nucleotides (adenosine 5′-triphosphate (ATP) and uridine 5′-triphosphate (UTP)) are well known to exert physiological effects on the cardiovascular system, whether nucleotides participate functionally in embryonic heart development is not clear. The responsiveness of embryonic cardiomyocytes (E) 12 to P2 receptor agonists by measuring Ca$^{2+}$ influx did not present response to ATP, but responses to P2 agonists were detected in cardiomyocytes taken from E14 and E18 rats. Photometry revealed that the responses to ATP were concentration-dependent with an EC$_{50}$ of 1.32 μM and 0.18 μM for E14 and E18 cardiomyocytes, respectively. In addition, other P2 agonists were also able to induce Ca$^{2+}$ mobilization. RT-PCR showed the presence of P2X2 and P2X4 receptor transcripts on E14 cardiomyocytes with a lower expression of P2X3 and P2X7 receptors. P2X1 and a low level of P2X5 receptor messenger RNA (mRNA) were also expressed at E18. Immunofluorescence data indicated that only P2X2 and P2X4 receptor proteins were expressed in E14 cardiomyocytes while protein for all the P2X receptor subtypes was expressed in E18, except for P2X3 and P2X6. Responses mediated by agonists specific for P2Y receptors subtypes showed that P2Y receptors (P2Y$_{1}$, P2Y$_{2}$, P2Y$_{4}$ and P2Y$_{6}$) were also present in both E14 and E18 cardiomyocytes. Dye transfer experiments showed that ATP induces coupling of cells at E12, but this response is decreased at E14 and lost at E18. Conversely, UTP induced coupling with five or more cells in most cells from E12 to E18. Our results show that specific P2 receptor subtypes are present in embryonic rat cardiomyocytes, including P2X7 and P2Y$_{4}$ receptors that have not been identified in adult rat cardiomyocytes. The responsiveness to ATP stimulation even before birth, suggests that ATP may be an important messenger in embryonic as well as in adult hearts.
Starting Page	127
Ending Page	138
Page Count	12
File Format	PDF
ISSN	15739538
Journal	Purinergic Signalling
Volume Number	11
Issue Number	1
e-ISSN	15739546
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-12-16
Publisher Institution	International Purine Club
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	ATP P2X receptors P2Y receptors P1 receptors Rat embryo Heart Cardiomyocyte FLIPR Calcium mobilization Biomedicine general Pharmacology/Toxicology Human Physiology Neurosciences Cancer Research
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

2′, 3′-O-(4-benzoylbenzoyl)–ATP-mediated calcium signaling in rat glioma C6 cells: role of the P2Y$_{2}$ nucleotide receptor

P2 receptors in cardiovascular regulation and disease ( Purinergic Signalling , Volume 4 , Issue 1 )

An evolutionary history of P2X receptors

Pharmacological and molecular characterization of functional P2 receptors in rat embryonic cardiomyocytes

P2 receptors in cardiovascular regulation and disease

Development of a comprehensive set of P2 receptor pharmacological research compounds

Involvement of P2X and P2Y receptors in microglial activation in vivo

Purinergic modulation of microglial cell activation

P2 receptors and platelet function

Pharmacological and molecular characterization of functional P2 receptors in rat embryonic cardiomyocytes

Similar Documents

2′, 3′-O-(4-benzoylbenzoyl)–ATP-mediated calcium signaling in rat glioma C6 cells: role of the P2Y$_{2}$ nucleotide receptor

P2 receptors in cardiovascular regulation and disease ( Purinergic Signalling , Volume 4 , Issue 1 )

An evolutionary history of P2X receptors

Pharmacological and molecular characterization of functional P2 receptors in rat embryonic cardiomyocytes

P2 receptors in cardiovascular regulation and disease

Development of a comprehensive set of P2 receptor pharmacological research compounds

Involvement of P2X and P2Y receptors in microglial activation in vivo

Purinergic modulation of microglial cell activation

P2 receptors and platelet function

Pharmacological and molecular characterization of functional P2 receptors in rat embryonic cardiomyocytes