NDLI: Catalytic characteristics of plant-esterase from wheat flour

Content Provider	Springer Nature Link
Author	Hou, Chang jun He, Kun Yang, Li min Huo, Dan qun Yang, Mei Huang, Shun Zhang, Liang Shen, Cai hong
Copyright Year	2011
Abstract	A plant-esterase extracted from wheat flour and purified with a PEG1000/NaH$_{2}$PO$_{4}$ aqueous two-phase system was characterized for its catalytic characteristics. The optimal condition for plant-esterase to catalyze 1-naphthyl acetate was at 30°C, pH 6.5. It kept stability at 20°C during 120 min and at pH 5.5 during 60 h. The effects of metal ions, chemical modification reagents and pesticides on plant-esterase activity were investigated. It was found that Ba$^{2+}$ and Pb$^{2+}$ at concentrations of 20 mM significantly inhibited the activity of plant-esterase while Mg$^{2+}$, Ca$^{2+}$ and Fe$^{2+}$ at the same concentration enhanced the enzyme activity. Chemical modification reagents significantly influenced the activity of plant-esterase. Particularly, PMSF (4.5 mM) and N-bromosuccinimide (11 mM) inhibited by 5.40–19.87% of the enzyme activity. It is implied that serine and tryptophan are related to the enzyme activity. Plant-esterase were displayed concentration-dependent inhibition by dichlorvos, carbofuran and carbendazim (IC50 = 0.31–63.12 ppm). All these results indicated that catalytic efficiency of plant-esterase strongly depends on reaction conditions, activity effectors and amino acid residues at the active site. It makes meaningful guidance on further design of sensing material in monitoring pesticides.
Starting Page	541
Ending Page	548
Page Count	8
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	28
Issue Number	2
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-08-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant- esterase Characterization Organophosphates Carbamates Biotechnology Microbiology Biochemistry Applied Microbiology Environmental Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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