NDLI: Thermostable invertases from Paecylomyces variotii produced under submerged and solid-state fermentation using agroindustrial residues

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Thermostable invertases from Paecylomyces variotii produced under submerged and solid-state fermentation using agroindustrial residues

Content Provider	Springer Nature Link
Author	Giraldo, Marielle Aleixo da Silva, Tony Márcio Salvato, Fernanda Terenzi, Héctor Francisco Jorge, João Atílio Guimarães, Luis Henrique Souza
Copyright Year	2011
Abstract	The filamentous fungus Paecylomices variotii was able to produce high levels of cell extract and extracellular invertases when grown under submerged fermentation (SbmF) and solid-state fermentation, using agroindustrial products or residues as substrates, mainly soy bran and wheat bran, at 40°C for 72 h and 96 h, respectively. Addition of glucose or fructose (≥1%; w/v) in SbmF inhibited enzyme production, while the addition of 1% (w/v) peptone as organic nitrogen source enhanced the production by 3.7-fold. However, 1% (w/v) (NH$_{4}$)$_{2}$HPO$_{4}$ inhibited enzyme production around 80%. The extracellular form was purified until electrophoretic homogeneity (10.5-fold with 33% recovery) by DEAE-Fractogel and Sephacryl S-200 chromatography. The enzyme is a monomer with molecular mass of 102 kDa estimated by SDS–PAGE with carbohydrate content of 53.6%. Optima of temperature and pH for both, extracellular and cell extract invertases, were 60°C and 4.0–4.5, respectively. Both invertases were stable for 1 h at 60°C with half-lives of 10 min at 70°C. Mg$^{2+}$, Ba$^{2+}$ and Mn$^{2+}$ activated both extracellular and cell extract invertases from P. variotii. The kinetic parameters K$_{m}$ and V$_{max}$ for the purified extracellular enzyme corresponded to 2.5 mM and 481 U/mg prot$^{−1}$, respectively.
Starting Page	463
Ending Page	472
Page Count	10
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	28
Issue Number	2
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-07-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Invertase β-Fructofuranosidase Sucrose Paecylomyces Fungi Biotechnology Microbiology Biochemistry Applied Microbiology Environmental Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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