NDLI: Applying MAR Analysis to Identify Human and Non-Human Fecal Sources in Small Kentucky Watersheds

Content Provider	Springer Nature Link
Author	Ritchey, S. A. Coyne, M. S.
Copyright Year	2008
Abstract	The recurrence of reports citing water quality impairments in watersheds is evidence that tools are needed to identify pollution sources and facilitate restoration efforts such as implementing total maximum daily limits (TMDLs) or best management practices (BMPs). Fecal bacteria in surface waters are one of the most commonly cited impairments to water quality. This study evaluated microbial source tracking (MST), specifically multiple antibiotic resistance (MAR) analysis, as a management tool to differentiate nonpoint source pollution into source groups. A library containing Escherichia coli (E. coli, EC) and fecal streptococci (FS) isolates from poultry (EC n = 282, FS n = 650), human (EC n = 152, FS n = 240), wildlife (EC n = 17, FS n = 43), horse (EC n = 79, FS n = 82), dairy cattle (EC n = 38, FS n = 42), and beef cattle (EC n = 49, FS n = 46) sources was created. The MAR analysis was conducted on the isolates using a profile of seven antibiotics. The antibiotic signatures of unknown source isolates from Elkhorn and Hickman Creek watersheds were evaluated against the library to determine the contributions of potential fecal inputs from the respective sources. Correct classification was >60% when analyzed at the human and non-human-level of classification. On a watershed basis, both watersheds produced similar results; inputs from non-human sources were the greatest contributors to nonpoint source pollution. The results from the multiple antibiotic resistance (MAR) analysis revealed that the information produced, coupled with knowledge of the watershed and its associated land uses, would be helpful in allocating resources to remediate impaired water quality in such watersheds.
Starting Page	115
Ending Page	125
Page Count	11
File Format	PDF
ISSN	00496979
Journal	Water, Air, and Soil Pollution
Volume Number	196
Issue Number	1-4
e-ISSN	15732932
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-06-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Waste management Fecal coliforms Fecal streptococci Biosource tracking Escherichia coli Watershed management Hydrogeology Terrestrial Pollution Waste Water Technology Water Pollution Control Water Management Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution Environment
Content Type	Text
Resource Type	Article
Subject	Ecological Modeling Environmental Chemistry Pollution Environmental Engineering Water Science and Technology

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