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| Content Provider | Springer Nature Link |
|---|---|
| Author | More Acosta, Pablo Mola, Mónica Huertas, Antonio Gómez, Myriam Sánchez Romero, Alfredo González, Mauricio Mercedes Bravo, María García Carrancá, Alejandro |
| Copyright Year | 2008 |
| Abstract | Human Papillomavirus type 16 (HPV 16) DNA is regularly found in around 50% of all cervical carcinomas. Variants of this type have been found associated with different risks for cervical cancer development. Presence of HPV 16 variants in Colombia has not been previously reported. The aims of this study were to assess the feasibility of non-radioactive PCR-SSCP (polymerase chain reaction single-strand conformation polymorphism) analysis for determination of variability of ORF of E6, variability in the enhancer sequence of the LCR, and for establishment of the distribution of HPV 16 variants in invasive squamous cell carcinoma of the uterine cervix in Colombian women. Biopsies from 59 patients at the Instituto Nacional de Cancerología (INC) in Bogotá (Colombia) were collected. HPV detection was performed using universal primers. HPV 16 variants were detected by non-radioactive single-stranded conformational polymorphism (SSCP) analysis and direct sequencing. HPV 16 was detected in 57.6% of the tumors. The European branch was identified in 88.2% of the samples with the E-G350 class being the most prevalent variant (41.1%). The Asian-American branch was identified in 8.8% of the samples. Within this group it was possible to distinguish between c and a classes. It was not possible to determine the branch in 2.9% of the cases. A nucleotide transition (G to A) at position 7521 was the most prevalent variation (80%) found in the enhancer sequence of the LCR region. Conclusion: A non-radioactive PCR-SSCP analysis allowed us to distinguish between European and Asian-American branches of HPV 16, and to distinguish among classes in squamous cell carcinomas of the uterine cervix in Colombia. This method is an excellent alternative that can be used as a screening tool for identification of HPV 16 variants. |
| Starting Page | 22 |
| Ending Page | 30 |
| Page Count | 9 |
| File Format | |
| ISSN | 09208569 |
| Journal | Virus Genes |
| Volume Number | 37 |
| Issue Number | 1 |
| e-ISSN | 1572994X |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2008-05-30 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | HPV 16 Variants PCR-SSCP Cervical cancer Colombia Plant Sciences Virology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Genetics Medicine Molecular Biology |
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