NDLI: Partial sequence analysis of VP1 of Indian isolates of foot-and-mouth disease virus type Asia-1

Content Provider	Springer Nature Link
Author	Rana, Samir Kumar Bagchi, Tamishraha
Copyright Year	2008
Abstract	Nucleotide sequence of 3′ end of VP1 (1D region) was determined using RT-PCR amplified DNA of 31 foot and mouth disease virus (FMDV) type Asia-1 field isolates originating from 11 different geographically distinct states of India during the period 1987–2000. These field strains exhibited an average of 7.5% divergence among them and were found to be divergent from the Indian vaccine strains Asia-1 WBN 117/85, IND 8/79, and IND 63/72, by an average 5.9, 14.8, and 7.4% divergence, respectively. Phylogenetic analysis of these 31 field isolates including 3 of the vaccine strains of India and sequences of 22 Indian field isolates obtained from the GenBank revealed that all the Indian FMDV type Asia-1 isolates belonged to a single genotype comprising of two distinct lineages (Lineages A and B). All the field isolates under study belonged to the Lineage-B comprising 8 different clusters, which also includes the vaccine strains WBN-117/85 and IND 8/79. Surprisingly, another vaccine strain IND 63/72 formed Lineage-A. Phylogenetic analysis of sequences of another 23 exotic type Asia-1 isolates from 15 different countries obtained from the GenBank along with the 56 Indian isolates revealed the existence of three distinct genotypes. The prototype strain Asia-1 PAK 1/54 belongs to a separate genotype. Two strains from India along with one strain each from China and Russia belongs to another genotype. The third genotype is formed by the remaining isolates including all the 31 isolates from the present study and exotic viruses from 14 other different countries. Comparison of deduced amino acid (aa) sequence indicated that majority of the mutations were found within two distinct regions corresponding to amino acid positions 130–160 and 193–211. The motif at aa positions 138–141 in vaccine strains WBN 117/85, IND 8/79 and in all the field isolates was ETTS/P; however, the same motif in IND 63/72 was TQPT. The motif 153–156 in majority of Indian isolates including vaccine strains WBN 117/85 and IND 8/79 was LSGQ/R whereas the same motif seen in IND 63/72 was VSNR. The study revealed that the FMDV type Asia-1 isolates circulating in the country are not highly heterogeneous, but showed considerable genetic variations. Certain mutations were also observed in the residues, which have been proved to be contributing to the formation of neutralizing epitopes. In neutralization studies employing polyclonal antisera, type Asia-1 WBN 117/85 revealed broader serological spectrum than other vaccine strains of India used in this study.
Starting Page	60
Ending Page	68
Page Count	9
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	37
Issue Number	1
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2008-05-30
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	FMDV Serotype Asia-1 1D region Plant Sciences Virology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Molecular relationships between type Asia 1 new strain from China and type O Panasia strains of foot-and-mouth-disease virus

Comparative complete genome analysis of Indian type A foot-and-mouth disease virus field isolates

Immunization of DNA vaccine encoding C3d-VP1 fusion enhanced protective immune response against foot-and-mouth disease virus

Molecular Characterization of Foot-and-mouth Disease Virus in Hong Kong During 2001–2002

Antigenic Variation in Foot and Mouth Disease Virus Type Asia 1 Isolates Circulated During 1993–95 in India

Capsid Protein-Encoding (P1) Sequence of Foot and Mouth Disease Virus Type Asia 1 Ind 63/72

A mutant of infectious Asia 1 serotype foot-and-mouth disease virus with the deletion of 10-amino-acid in the 3A protein

Comparative sequence analysis of representative foot-and-mouth disease virus genomes from Southeast Asia

Genetic characterization of a new pandemic Southeast Asia topotype strain of serotype O foot-and-mouth disease virus isolated in China during 2010

Partial sequence analysis of VP1 of Indian isolates of foot-and-mouth disease virus type Asia-1

Similar Documents

Molecular relationships between type Asia 1 new strain from China and type O Panasia strains of foot-and-mouth-disease virus

Comparative complete genome analysis of Indian type A foot-and-mouth disease virus field isolates

Immunization of DNA vaccine encoding C3d-VP1 fusion enhanced protective immune response against foot-and-mouth disease virus

Molecular Characterization of Foot-and-mouth Disease Virus in Hong Kong During 2001–2002

Antigenic Variation in Foot and Mouth Disease Virus Type Asia 1 Isolates Circulated During 1993–95 in India

Capsid Protein-Encoding (P1) Sequence of Foot and Mouth Disease Virus Type Asia 1 Ind 63/72

A mutant of infectious Asia 1 serotype foot-and-mouth disease virus with the deletion of 10-amino-acid in the 3A protein

Comparative sequence analysis of representative foot-and-mouth disease virus genomes from Southeast Asia

Genetic characterization of a new pandemic Southeast Asia topotype strain of serotype O foot-and-mouth disease virus isolated in China during 2010

Partial sequence analysis of VP1 of Indian isolates of foot-and-mouth disease virus type Asia-1