NDLI: Characterization of the murine cytomegalovirus m136 gene

Content Provider	Springer Nature Link
Author	Visalli, Robert J. Nicolosi, Denise M. Irven, Karen Khan, Tamseel Visalli, Melissa A.
Copyright Year	2006
Abstract	The 230-kbp murine cytomegalovirus (MCMV) genome is predicted to encode 182 open reading frames (orfs). One gene whose functional role is not known is encoded by the 762-bp m136 orf. Sequence analysis of rat cytomegalovirus (RCMV) strains Maastricht and English revealed homologous orfs, pr136, and ORF HJ4, respectively. Conservation of these orfs suggested that m136 and the RCMV homologs might play a role during virus replication. Expression of an epitope tagged form of m136 (m136-V5) yielded a polypeptide of 34 kDa that localized to the perinuclear region of transfected mouse 3T3 fibroblasts. Three independently generated MCMV m136 mutants were isolated and characterized. Mutations were introduced into the m136 orf by inserting either a β-glucuronidase (m136-β-gluc) or a guanosine phosphoribosyl transferase (m136-gpt) expression cassette into a unique BglII site, or by inserting a gpt cassette into a deleted region (Δm136) of m136. No differences were observed in viral yield, plaque size, and plaque morphology between the parental strain and any of the m136 mutant viruses. In vivo analysis using a SCID mouse virulence model showed a consistently measurable attenuated phenotype for all three m136 mutants. The results showed that although the m136 gene was not essential for replication in vitro or in vivo, an intact m136 gene was necessary to yield wild type virulence during infection of the host.
Starting Page	117
Ending Page	126
Page Count	10
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	34
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-12-02
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Murine cytomegalovirus m136 orf m136 gene m136 mutants Plant Sciences Virology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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