NDLI: Identification and characterization of Orf virus 050 protein proteolysis

Content Provider	Springer Nature Link
Author	Wang, Han Jiang, Junyi Ding, Rui Wang, Xiaoping Liao, Meiying Shao, Jing Luo, Xiaohong Luo, Shuhong Duan, Chaohui
Copyright Year	2017
Abstract	The Orf virus 050 (ORFV050) gene is located in the core region of the ORFV genome. It is similar to Vaccinia virus (VV) Copenhagen L4R, and encodes the DNA-binding virion core protein VP8, which has structures similar to the VV P25K core protein and may undergo similar proteolytic processing during virus assembly. Three conserved Ala–Gly–X motifs at putative cleavage sites were identified in ORFV050. To investigate the proteolysis of ORFV050 and its participation in viral assembly, full-length and site-directed mutant ORFV050 recombinant proteins were constructed and expressed. Two distinct protein bands of 28.5 and 25 kDa were detected in the infected cells using anti-ORFV050 polyclonal antiserum. A potential cleavage site was identified at amino acids 30–32 of ORFV050. Mutation of AG/A to (R) in ORFV050 abolished the process of proteolysis. ORFV050 is a late gene synthesized during viral replication in the host cytoplasm. According to these results, we conclude that ORFV050 undergoes proteolysis and plays an important role in viral assembly.
Starting Page	400
Ending Page	409
Page Count	10
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	53
Issue Number	3
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2017-02-11
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Orf virus ORFV050 Cleavage site Polyclonal antiserum Proteolysis Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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