NDLI: Identification and characterization of host cell proteins interacting with Scylla serrata reovirus non-structural protein p35

Content Provider	Springer Nature Link
Author	Yuan, Yangyang Fan, Dongyang Zhu, Sidong Yang, Jifang Chen, Jigang
Copyright Year	2016
Abstract	We have previously shown that non-structural protein p35, encoded by Scylla serrata reovirus (SsRV) S10, may act as a viroporin. To characterize the role of p35 protein in the modulation of cellular function, a yeast two-hybrid system was used to screen a cDNA library derived from S. serrata to find its interacting partner. Protein interactions were confirmed in vitro by GST pull-down. Full cDNAs of p35 interactors were cloned by the rapid amplification of cDNA ends. After two-hybrid library screening, we isolated partial cDNAs encoding hemocyanin, cryptocyanin, and TAX1BP1. Interaction of p35 with each of hemocyanin, cryptocyanin, and TAX1BP1 was confirmed by GST pull-down. The full-length cDNA fragments of hemocyanin, cryptocyanin, and TAX1BP1 were 2287, 2422, and 3437 bp, respectively, and they encoded three putative proteins with molecular masses of ~76.9, ~79.2, and ~107.2 kDa, respectively. This study casts new light on the function and physiological significance of p35 during the SsRV replication cycle.
Starting Page	317
Ending Page	322
Page Count	6
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	53
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2016-12-09
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Scylla serrata reovirus (SsRV) Yeast two-hybrid system Hemocyanin Cryptocyanin TAX1BP1 Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Identification and RNA segment assignment of six structural proteins of Scylla serrata reovirus

Scylla serrata reovirus p35 protein expressed in Escherichia coli cells alters membrane permeability

Molecular characterization of eight segments of Scylla serrata reovirus (SsRV) provides the complete genome sequence

Human heart cell proteins interacting with a C-terminally truncated 2A protein of coxsackie B3 virus: identification by the yeast two-hybrid system

Identification and screening of host proteins interacting with ORFV-ORF047 protein

Screening and Identification of the Host Proteins Interacting with Toxoplasma gondii Rhoptry Protein ROP16

Identification of the Novel Host Protein Interacting With the Structural Protein VP1 of Chinese Sacbrood Virus by Yeast Two-Hybrid Screening

Identification of host proteins, Spata3 and Dkk2, interacting with Toxoplasma gondii micronemal protein MIC3

Protein–protein interactions between proteins of Citrus tristeza virus isolates

Identification and characterization of host cell proteins interacting with Scylla serrata reovirus non-structural protein p35

Similar Documents

Identification and RNA segment assignment of six structural proteins of Scylla serrata reovirus

Scylla serrata reovirus p35 protein expressed in Escherichia coli cells alters membrane permeability

Molecular characterization of eight segments of Scylla serrata reovirus (SsRV) provides the complete genome sequence

Human heart cell proteins interacting with a C-terminally truncated 2A protein of coxsackie B3 virus: identification by the yeast two-hybrid system

Identification and screening of host proteins interacting with ORFV-ORF047 protein

Screening and Identification of the Host Proteins Interacting with Toxoplasma gondii Rhoptry Protein ROP16

Identification of the Novel Host Protein Interacting With the Structural Protein VP1 of Chinese Sacbrood Virus by Yeast Two-Hybrid Screening

Identification of host proteins, Spata3 and Dkk2, interacting with Toxoplasma gondii micronemal protein MIC3

Protein–protein interactions between proteins of Citrus tristeza virus isolates

Identification and characterization of host cell proteins interacting with Scylla serrata reovirus non-structural protein p35