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| Content Provider | Springer Nature Link |
|---|---|
| Author | Bozorgi, Naghmeh Heydarnejad, Jahangir Kamali, Mehdi Massumi, Hossain |
| Copyright Year | 2016 |
| Abstract | Beet curly top Iran virus (BCTIV) is a distinct geminivirus which has been reported from sugar-beet-growing farms in Iran. In this study, the role of the splicing in expression of complementary-sense genes of BCTIV was studied. Total RNA was extracted from BCTIV-infected tissue, and the predicted intron position of complementary-sense mRNA transcripts was amplified by RT-PCR followed by cloning of the amplicons. Sequence confirmed that both spliced and unspliced mRNAs are synthesized by the same transcription unit. Sequence comparison showed that a 155-nt segment (intron) corresponding to nucleotides 1890–2044 of the viral genome has been removed from the latter transcript and therefore fusion of the C1:C2 genes resulted creation of a continuous reading frame for potential production of intact replication initiator protein (Rep). BCTIV intron comprises of most consensus splicing signals required for splicing in eukaryotes and several plant viruses including mastre- and capulaviruses. |
| Starting Page | 323 |
| Ending Page | 327 |
| Page Count | 5 |
| File Format | |
| ISSN | 09208569 |
| Journal | Virus Genes |
| Volume Number | 53 |
| Issue Number | 2 |
| e-ISSN | 1572994X |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2016-12-21 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Splicing Beet curly top Iran virus Becurtovirus Geminivirus Intron Medical Microbiology Virology Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Genetics Medicine Molecular Biology |
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