NDLI: Protein–protein interactions between proteins of Citrus tristeza virus isolates

Content Provider	Springer Nature Link
Author	Nchongboh, Chofong Gilbert Wu, Guan wei Hong, Ni Wang, Guo ping
Copyright Year	2014
Abstract	Citrus tristeza virus (CTV) is one of the most devastating pathogens of citrus. Its genome is organized into 12 open reading frames (ORFs), of which ten ORFs located at the 3′-terminus of the genome have multiple biological functions. The ten genes at the 3′-terminus of the genome of a severe isolate (CTV-S4) and three ORFs (CP, CPm and p20) of three other isolates (N4, S45 and HB1) were cloned into pGBKT7 and pGADT7 yeast shuttle vectors. Yeast two-hybridization (Y2H) assays results revealed a strong self-interaction for CP and p20, and a unique interaction between the CPm of CTV-S4 (severe) and CP of CTV-N4 (mild) isolates. Bimolecular fluorescence complementation also confirmed these interactions. Analysis of the deletion mutants delineated the domains of CP and p20 self-interaction. Furthermore, the domains responsible for CP and p20 self-interactions were mapped at the CP amino acids sites 41–84 and p20 amino acids sites 1–21 by Y2H. This study provided new information on CTV protein interactions which will help for further understanding the biological functions.
Starting Page	456
Ending Page	465
Page Count	10
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	49
Issue Number	3
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2014-07-27
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Citrus tristeza virus Protein interaction Yeast two-hybrid Bimolecular fluorescence complementation (BiFC) Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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