NDLI: Screening pigs for xenotransplantation: expression of porcine endogenous retroviruses in transgenic pig skin

Content Provider	Springer Nature Link
Author	Kimsa Dudek, Magdalena Strzalka Mrozik, Barbara Kimsa, Malgorzata W. Blecharz, Irena Gola, Joanna Skowronek, Bartlomiej Janiszewski, Adrian Lipinski, Daniel Zeyland, Joanna Szalata, Marlena Slomski, Ryszard Mazurek, Urszula
Copyright Year	2015
Abstract	Pigs seem to be the answer to worldwide organ donor shortage. Porcine skin may also be applied as a dressing for severe burns. Genetic modifications of donor animals enable reduction of immune response, which prolongs xenograft survival as temporary biological dressing and allows achieving resistance against xenograft rejection. The risk posed by porcine endogenous retroviruses (PERVs) cannot be eliminated by breeding animals under specific-pathogen-free conditions and so all recipients of porcine graft will be exposed to PERVs. Therefore our study has been focused on the assessment of PERV DNA and mRNA level in skin samples of transgenic pigs generated for xenotransplantation. Porcine skin fragments were obtained from 3- to 6-month-old non-transgenic and transgenic Polish Landrace pigs. Transgenic pigs were produced by pronuclear DNA microinjection and were developed to express the human α-galactosidase and the human α-1,2-fucosyltransferase gene. The copy numbers of PERV DNA and RNA were evaluated using real-time Q-PCR and QRT-PCR. Comparative analysis of all PERV subtypes revealed that PERV-A is the main subtype of PERVs in analyzed skin samples. There was no significantly different copy number of PERV-A, PERV-B and PERV-C between non-transgenic pigs, pigs with the human α-galactosidase and pigs expressing the human α-1,2-fucosyltransferase gene, except of PERV-C DNA. It brings the conclusion, that transgenesis process exerts no influence on PERVs transinfection. That is another step forward in the development of pig skin xenografts as burn wounds dressing.
Starting Page	529
Ending Page	536
Page Count	8
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	24
Issue Number	3
e-ISSN	15739368
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-03-27
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Porcine endogenous retroviruses Xenotransplantation Transgenic pigs QPCR QRT-PCR Molecular Medicine Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Transgenics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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