NDLI: The aflatoxin-detoxifizyme specific expression in mouse parotid gland

Content Provider	Springer Nature Link
Author	Guan, Li zeng Sun, Yu ping Cai, Jin shun Wu, Han dong Yu, Long zheng Zhang, Yong liang Xi, Qian yun
Copyright Year	2015
Abstract	The aflatoxin-detoxifizyme (ADTZ) gene derived from Armillariella tabescens was cloned into parotid gland-specific expression vector (pPSPBGPneo) to construct the parotid gland-specific vector expressing ADTZ (pPSPBGPneo-ADTZ). Transgenic mice were generated by microinjection and identified by using PCR and Southern blotting analysis. PCR and Southern blotting analysis showed that total six transgenic mice carried the ADTZ gene were generated. RT-PCR analysis indicated that the expression of ADTZ mRNA could be detected only in parotid glands of the transgenic mice. The ADTZ activity in the saliva was found to be 3.72 ± 1.64 U/mL. After feeding a diet containing aflatoxin B1 (AFB1) for 14 days, the effect of ADTZ on serum biochemical indexes and AFB1 residues in serum and liver of mice were evaluated. The results showed that total protein and globulin contents in the test treatment (transgenic mice) produced ADTZ were significantly higher than that of the positive control, while alanine aminotransferase and aspartate aminotransferase activity in serum of the test treatment (transgenic mice) were remarkably lower compared to that of the positive control (P < 0.05). Moreover, AFB1 residues in serum and liver of the test treatment (transgenic mice) were significantly lower compared with that of the positive control (P < 0.05). These results in the study confirmed that ADTZ produced in transgenic mice could reduce, even eliminate the negative effects of AFB1 on mice.
Starting Page	489
Ending Page	496
Page Count	8
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	24
Issue Number	3
e-ISSN	15739368
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-01-21
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Parotid gland Aflatoxin-detoxifizyme Transgenic mice Molecular Medicine Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Transgenics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

β-Glucanase specific expression in the parotid gland of transgenic mice

Improvement of anti-nutritional effect resulting from β-glucanase specific expression in the parotid gland of transgenic pigs

Skeletal defects in Osterix-Cre transgenic mice

Ectopic expression of tethered human follicle-stimulating hormone (hFSH) gene in transgenic mice

Swine PPAR-γ2 expression upregulated in skeletal muscle of transgenic mice via the swine Myozenin-1 gene promoter

Muscle-specific transgenic expression of porcine myostatin propeptide enhances muscle growth in mice

Generation of mice encoding a conditional null allele of Gcm2

Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays)

Functional verification of a porcine myostatin propeptide mutant

The aflatoxin-detoxifizyme specific expression in mouse parotid gland

Similar Documents

β-Glucanase specific expression in the parotid gland of transgenic mice

Improvement of anti-nutritional effect resulting from β-glucanase specific expression in the parotid gland of transgenic pigs

Skeletal defects in Osterix-Cre transgenic mice

Ectopic expression of tethered human follicle-stimulating hormone (hFSH) gene in transgenic mice

Swine PPAR-γ2 expression upregulated in skeletal muscle of transgenic mice via the swine Myozenin-1 gene promoter

Muscle-specific transgenic expression of porcine myostatin propeptide enhances muscle growth in mice

Generation of mice encoding a conditional null allele of Gcm2

Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays)

Functional verification of a porcine myostatin propeptide mutant

The aflatoxin-detoxifizyme specific expression in mouse parotid gland