NDLI: Thidiazuron induces high-frequency indirect shoot organogenesis of Bienertia sinuspersici: a single-cell C$

Content Provider	Springer Nature Link
Author	rthmore, Jennifer Anne Sigurdson, Dustin Schoor, Sarah Rustum, Amer Chuong, Simon D. X.
Copyright Year	2016
Abstract	Induction of high-frequency shoot organogenesis using stem-derived callus of Bienertia sinuspersici was achieved on Murashige and Skoog basal medium containing 0.0045–9.0 μM thidiazuron (TDZ). Other cytokinins [kinetins, and 6-benzyladenine (BA)] were also tested for their shoot induction potential but only TDZ was effective at promoting shoot morphogenesis and development. The highest shoot induction efficiency (80 %) was obtained on a medium containing 0.45 μM TDZ. The TDZ-derived shoots were transferred to medium containing 8.8 μM BA for multiplication followed by gibberellic acid (1.45–14.5 μM) treatment to promote shoot elongation. The elongated shoots spontaneously developed adventitious roots during the elongation process. The rooted plantlets were successfully transplanted to soil, acclimated in the greenhouse and then transferred to a growth chamber under controlled conditions. Histological analysis of leaf anatomy of the regenerated plants showed that the distinctive cytoplasmic compartmentation feature of the single-cell C$_{4}$ photosynthetic system was re-established. Moreover, Western blots detected high levels of key C$_{4}$ enzymes in mature leaves of TDZ-derived shoots in vitro and the regenerated plants. Overall, these findings show that in vitro regenerated plants possessed the single-cell C$_{4}$ leaf anatomy and C$_{4}$ enzymes comparable to those of the seed-derived plants.
Starting Page	141
Ending Page	151
Page Count	11
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	126
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-03-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bienertia sinuspersici Indirect organogenesis Single-cell C$_{4}$ photosynthesis Regeneration Thidiazuron Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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