NDLI: Downregulation of the mitogen-activated protein kinase SlMAPK7 gene results in pollen abortion in tomato

Content Provider	Springer Nature Link
Author	Chen, Lifei Guan, Xiaoyan Qin, Li Zou, Tao Zhang, Youwei Wang, Jie Wang, Yan Pan, Changtian Lu, Gang
Copyright Year	2016
Abstract	Mitogen-activated protein kinases (MAPKs) play important roles in various aspects including plant development and stress response. The biological role of MAPKs in tomato development remains unclear. Here, multiple stable transgenetic lines were developed using either gene overexpression or RNA interference (RNAi) technology to investigate the role of SlMAPK7 in tomato reproductive development. Either upregulation or downregulation of SlMAPK7 did not drastically disturb the vegetative growth of tomato. However, in RNAi-based gene silencing plants, approximately 90 % of the pollen grains were identified to be abnormal and lack viability, which were malformed or collapsed. Cytological observations showed that anthers could develop normal microspore mother cells, but the uninucleate microspores failed to form normal mature pollen grains. Transmission electron microscopy examination demonstrated that accelerated degradation of tapetum occurred in the anther and finally the intine layer of pollen appears abnormal. In addition, although inhibition of SlMAPK7 gene did not alter fruit morphology, seed number in RNAi fruits was markedly reduced. Thus, the results demonstrated that SlMAPK7 is involved in tomato pollen development, possibly via regulation the degradation of tapetum.
Starting Page	79
Ending Page	92
Page Count	14
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	126
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-03-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Solanum lycopersicum Mitogen-activated protein kinases SlMAPK7 Pollen development RNAi Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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