NDLI: Hairy root cultures of Panax vietnamensis, a promising approach for the production of ocotillol-type ginsenosides

Content Provider	Springer Nature Link
Author	Ha, Loan Thi Pawlicki Jullian, Nathalie Pillon Lequart, Michelle Boitel Conti, Michèle Duong, Hoa Xo Gontier, Eric
Copyright Year	2016
Abstract	Panax vietnamensis Ha et Grushv. is an important source of majonoside R2, a dammarane saponin with an ocotillol side-chain, which is an interesting pharmacological active compound. Thirty-one transformed root lines were obtained after the inoculation of in vitro shoot explants of P. vietnamensis with Rhizobium rhizogenes ATCC 15834 strain. The transgenic nature of the hairy roots was confirmed by PCR amplification. The established hairy root lines displayed four morphological phenotypes when cultured on hormone-free medium. Most of the cultures showed the characteristic traits of hairy roots with primary roots, an extensive lateral branching and rapid growth. Kinetic growth of these hairy root lines was measured for 90 days and a biomass production of about 10 g L$^{−1}$ fresh weight was obtained. The ginsenoside content of hairy roots was identified by LC–MS and compared with that of 6 year-old rhizomes of P. vietnamensis. Majonoside R2 was recovered in trace amounts, but other ocotillol saponins, pseudoginsenoside F11 and vinaginsenoside R1, were recovered in larger proportions. These results indicate that hairy roots of P. vietnamensis present an interesting potential to produce ginsenosides and, in particular, ocotillol saponins.
Starting Page	93
Ending Page	103
Page Count	11
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	126
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-03-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Panax vietnamensis Transformed roots Rhizobium rhizogenes 15834 Ocotillol saponins Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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