NDLI: Transferability of rice SSR markers to bamboo

Content Provider	Springer Nature Link
Author	Chen, Shu Yun Lin, Yu Tsung Lin, Chia Wei Chen, Wei Yu Yang, Chih Hung Ku, Hsin Mei
Copyright Year	2010
Abstract	Simple sequence repeat (SSR) markers are widely applied in studies of plant molecular genetics due to their abundance in the genome, codominant nature, high repeatability, and transferability in cross-species applications. To investigate the possibility of applying rice SSR markers in bamboo, we selected 120 rice SSR markers that are evenly distributed on rice chromosomes and assessed these for their transferability to 21 different bamboo species. A total of 4847 bands of 2196 alleles were obtained from 82 SSR markers that were able to amplify products in the bamboo genome; the transferability was 68.3%. Seven markers specifically amplified individual bamboo species and are consequently valuable markers for species identification. SSR markers located on rice chromosome 7 and 1 showed the highest and lowest transferability, respectively to the bamboo genome. SSR markers located on some regions of the rice chromosomes could not be amplified in bamboo, suggesting that regional divergence occurred between rice and bamboo during evolution. A dendrogram was constructed. The dendrogram classified bamboo species into two major groups which coincided with rhizome type, runner, and clumper. The results of this study demonstrate that rice SSR markers can be a valuable source of markers for those genomes lacking useful marker systems.
Starting Page	23
Ending Page	33
Page Count	11
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	175
Issue Number	1
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-03-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Genetic diversity Genome Microsatellite Biotechnology Plant Physiology Plant Pathology Plant Genetics & Genomics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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