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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ivavskii, Yu. A. Kulepav, V. N. |
| Copyright Year | 2008 |
| Abstract | Zoospores of Laminaria at the stage of zoospore germination fixed to glass slides were irradiated by γ-rays in doses of 50, 100, or 250 Gy; or treated with colchicine at a concentration of 4 × 10$^{−5}$% for 5 days. The cultivation was conducted in vessels with seawater at a temperature of 12°N and illumination of 4000 lux for one month. Once a day, from day 22 to day 30, the temperature was reduced to 0°N for 12 h. As a result, in experimental samples gametophytes appeared that did not form gametangia; these appeared by the third day of cultivation, as plaques up to 2 cm in diameter (1–2 plaques per slide). In the same culture we found structures (1–2 per slide) consisting of strictly radially arranged rows of somatic cells attached to the slides. Later, these disks transformed into cones up to 0.5 cm in diameter. We recorded the development of a single-layered sporophyte of Laminaria arising from the center of such a cone. |
| Starting Page | 325 |
| Ending Page | 328 |
| Page Count | 4 |
| File Format | |
| ISSN | 10630740 |
| Journal | Russian Journal of Marine Biology |
| Volume Number | 34 |
| Issue Number | 5 |
| e-ISSN | 16083377 |
| Language | English |
| Publisher | SP MAIK Nauka/Interperiodica |
| Publisher Date | 2008-11-08 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | callus somatic embryogenesis Laminaria japonica cell cultures Freshwater & Marine Ecology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Aquatic Science Oceanography |
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