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| Content Provider | Springer Nature Link |
|---|---|
| Author | Liu, Fuli Wang, Feijiu Duan, Delin |
| Copyright Year | 2012 |
| Abstract | The public availability of numerous expressed sequence tag (EST) enables EST-based SSR (simple sequence repeat) markers to be widely used for genetics and breeding studies. In the present study, EST-SSR markers were developed from ESTs of Laminaria digitata and were transferred to the non-congeneric species Saccharina japonica. Among the 2,668 non-redundant ESTs, 83 (3.1%) ESTs containing SSR were identified totally, with an average of one SSR per 13.6 kb. Analysis of SSR motifs revealed that the trinucleotide and tetranucleotide were major motifs, accounted for 44.58% and 16.87%, respectively. Based on the 83 ESTs containing SSR, we designed 45 pairs of primers in the flanking regions of the SSR, of which 13 pairs showed polymorphism in a wild S. japonica population, and the mean alleles per locus was 3.6 (ranging from 2 to 6). The observed (Ho) and expected (He) heterozygosities of these EST-SSRs were 0.234–0.632 and 0.260–0.635, respectively. All loci were in Hardy–Weinberg equilibrium in the wild population and no linkage disequilibrium was detected among loci. The obtained EST-SSR markers can facilitate and promote related research such as ecological investigation, genetic diversity assessment and breeding practice of S. japonica as well. |
| Starting Page | 501 |
| Ending Page | 505 |
| Page Count | 5 |
| File Format | |
| ISSN | 09218971 |
| Journal | Journal of Applied Phycology |
| Volume Number | 24 |
| Issue Number | 3 |
| e-ISSN | 15735176 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2012-02-18 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Saccharina japonica Laminaria digitata EST SSR Phaeophyta Freshwater & Marine Ecology Plant Sciences Plant Physiology Ecology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Aquatic Science |
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