NDLI: Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)

Content Provider	Springer Nature Link
Author	Yao, Jianting Fu, Wandong Wang, Xiuliang Duan, Delin
Copyright Year	2008
Abstract	RNA isolation is difficult in some plants and algae because phenolics, polysaccharides, or other compounds can bind or co-precipitate with RNA, and because the success of RNA isolation can be strain-specific and species-specific. To create an improved RNA isolation protocol for Laminaria japonica Aresch (Laminariaceae, Phaeophyta), four methods for extracting RNA were tested. A cetyltrimethylammonium bromide (CTAB)-based RNA extraction protocol was developed that clearly showed 28S and 18S ribosomal RNA bands and produced RNA with high yield (68 μg g$^{−1}$ fresh weight) and high quality (A $_{260/280}$ ratio 1.96 ± 0.05). The isolated RNA was intact, and RT-PCR analysis confirmed that further molecular application is feasible.
Starting Page	233
Ending Page	238
Page Count	6
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	21
Issue Number	2
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-08-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Phaeophyta Phenolics CTAB Polysaccharides RNA extraction Ecology Plant Physiology Freshwater & Marine Ecology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)